子宫颈癌组织中水通道蛋白8和bcl-2蛋白的表达及其相关性

Expression of aquaporin-8 and bci-2 protein in human cervical carcinoma and their correlations

目的探讨水通道蛋白（AQP）8、bcl-2蛋白在宫颈癌组织中的表达及其相关性。方法采用免疫组化Envision二步法检测AQP8和bcl-2蛋白在74例宫颈癌（其中鳞癌46例、腺癌28例）、34例宫颈上皮内瘤变（CIN）和15例正常宫颈组织中的表达情况，并分析两者的相关性。结果AQP8和bcl-2蛋白主要在CIN异型细胞和宫颈癌细胞的细胞质内表达，AQP8蛋白在宫颈鳞癌、腺癌、CIN和正常宫颈组织中的阳性表达率分别为98％、61％、71％和53％，鳞癌高于腺癌、CIN和正常宫颈组织，差异有统计学意义（P〈0．01）；腺癌与CIN、正常宫颈组织比较，CIN与正常宫颈组织比较，差异均无统计学意义（P〉0．05）。bcl-2蛋白在宫颈鳞癌、腺癌、CIN和正常宫颈组织中的阳性表达率分别为74％、71％、53％和20％，鳞癌与腺癌组织比较，差异无统计学意义（P〉0．05）；鳞癌、腺癌高于CIN、正常宫颈组织，CIN也高于正常宫颈组织，差异均有统计学意义（P〈0．01）。AQP8和bcl-2蛋白在宫颈癌组织中的表达呈明显正相关（rs=0．463，P=0．000）。结论AQP8和bcl-2蛋白在宫颈癌组织中的表达呈明显正相关，AQP8蛋白表达上调可能与宫颈癌的发生、发展有一定的关系。

Objective To investigate the expression of aquaporin-8 （AQP8） and apoptosis associated bcl-2 protein in human cervical carcinoma and their relationship. Methods The expression of AQP8 and bcl-2 protein in 74 cases of cervical carcinoma （46 cases of squamous-cell carcinoma of the uterine cervix, 28 cases of adenocarcinoma of the uterine cervix ） , 34 cases of cervical intraepithelial neoplasia （CIN） and 15 cases of normal cervices were detected by immunohistochemical technique, and their clinical significance were analyzed. Results The expression of AQP8 and bcl-2 protein were detected in intracytoplasm of atypia cells in CIN, squamous-cell carcinoma and adenocarcinoma of the uterine cervix. The positive rates of AQP8 and bcl-2 in squamous-cell carcinoma, adenocarcinoma, CIN and normal cervical epithelium were 98%, 74% ; 61% , 71% ; 71% , 53% ; 53% ,20% respectively. There were significant differences between squamous-cell carcinoma of the uterine cervix and other groups in AQP8 （ P 〈 0. 01 ） , but no significant differences were found in any other groups. There were significant differences between squamous-cell carcinoma of the uterine cervix and CIN or normal cervical epithelium in bcl-2, so were between adenocarcinoma of the uterine cervix. The expression of AQP8 was positively correlated with bcl-2 in human cervical carcinoma（ rs = 0. 463, P = 0. 000 ）. Condusions There is a close relationship between high expression of AQP8 and development of human cervical carcinoma. The expression of AQP8 protein is positively correlated with bcl-2 protein in human cervical carcinoma. AQP8 protein may have anti-apoptosis function, although the detailed mechanism in human cervical carcinoma remains to be clarified.