氩氦靶向治疗诱导肺癌细胞凋亡及其相关蛋白的表达

Apoptosis and expression of related genes induced by argon-helium-based cryo-care system in lung carcinoma

目的探讨氩氦刀除冷冻直接杀伤肺癌细胞外的可能治疗机制。方法建立小鼠肺腺癌动物模型．氩氦刀冷冻后，末端标记法（TUNEL）原位检测细胞凋亡，免疫组织化学SABC法检查bcl-2和bax表达。结果在冷冻中心区，细胞死亡形成以坏死为主；在冷冻周边区，光镜形态学检查及TUNEL染色可见细胞凋亡，并有两次凋亡高峰出现。免疫组化结果显示．bcl-2蛋白表达在冷冻前后无明显变化，bax蛋白在冷冻后其表这上调。结论氩氦刀可以引起冷冻区周边部分细胞发生凋亡，其凋亡机制有bax蛋白参与，而与bcl-2无关。为增加冷冻疗效，术后可结合放化疗。

Objective To investigate the possible mechanism of killing lung carcinoma except direct necrosis induced by argon-helium-based cryocare system. Methods The experimental models of mouse with adenocarcinoma were established and then subjected to co, o-treatment via argon-helium-based cryocare system. The cell apoptosis in situ was examined with TUNEL staining. The expression of bcl-2 protein and bax protein was detected by immunohistochemichal procedures. Results In the center of the cryo-lesion, cell death occurred mainly by means of necrosis. But in the periphery of the cryo-lesion, cell death occurred mainly by means of apopotosis determined by morphological analysis and TUNEL staining, and twice high levels of apopotosis were observed. The expression of bax protein increased significantly. Meanwhile, the obvious changes for the expression of bcl-2 protein had not been observed. Conclusion The argon-helium-based cryocare system can induce cell apoptosis in the periphery of the cryolesion by upregulation of bax expression but not bcl-2 expression. And chemotherapy or radiotherapy can be combined to improve the cryosurgery efficiency.