人脐血和胎盘中肝细胞生长因子和纤维连接蛋白的测定

The detection of human HGF and Fn in the placenta and cord blood.

目的:检测人脐血、胎盘组织和胎盘基质细胞培养上清液中肝细胞生长因子(HGF)和纤维连接蛋白(Fn)的含量。方法:选择健康足月新生儿,分娩时留取脐血和胎盘组织,用ELISA法检测其中Fn和HGF的浓度,并比较分析。结果:HGF含量在脐血、胎盘组织提取液和胎盘基质细胞培养上清液中分别为1.23±0.24ng/ml、113.73±11.99ng/ml、2.23±0.31ng/ml。Fn含量分别为1105±44ng/ml、1537±319ng/ml、1027±171ng/ml。结论:与脐血相比,胎盘是提取HGF和Fn的优质材料,将其浓缩或纯化后具有很大的科研和应用价值。

Objective：To detect the concentration of hepatocyte growth factor（HGF） and fibronectin（Fn） in human placenta,cord blood and supematant of placental stroma cells. Method ： Human cord blood and placenta tissue were collected from normal newborn. The concentration of HGF and Fn were detected by ELISA. Result：The concentration of HGF in cord blood, placenta extraction and supematant of placental stroma cells were 1.23 ± 0.24 ng/ml, 113.73 ± 11.99ng/ml,2.23 ± 0.31ng/ml,while the concentration of Fn were 1105 ± 44ng/ml, 1537 ± 319ng/ml, 1027 ± 171ng/ml ,respectively. Conclusion： Compared to cord blood, placenta is good material for extract HGF and Fn with high value of research and application after purification.