血清胰岛素样生长因子-1影响小鼠肿瘤模型的实验研究

The effect of serum insulin like factor I on mouse cancer

目的探讨血清胰岛素样生长因子-1（IGF-1）水平对小鼠肿瘤模型的影响。方法用小鼠结肠腺癌CT26细胞接种于肝特异性IGF-1基因缺失鼠[LID]及对照组小鼠皮下，共120只6周龄的雄鼠接受了肿瘤移植，其中对照鼠和LID鼠各60只。2组小鼠分别又随机分成2个亚组（每个亚组各30只小鼠）。一个亚组每10只分别在肿瘤移植后第10，14，18天开始每天一次接受皮下注射重组人GH（1mg／kg）至第22天结束；另一个亚组接受盐水注射。结果接受GH注射的LID和对照鼠血清IGF-1均升高。从第10天开始干预时，高水平的IGF-1促进恶病质形成。从第14开始干预时，血清IGF-1水平与小鼠恶病质状态呈负相关。18d则效果不明显。结论肿瘤早期，IGF-1，GH加快肿瘤生长，促进恶病质形成。当恶病质已形成时，早期干预则可以改善之。

Objective To determine the effect of different serum insulin-like growth factor-I （IGF-1） levels on mouse cancer. Methods A total of 120 male mice at 6 weeks of age （60 control mice and 60 LID mice） were subcutaneously injected colon tumor CT26 cell line. Each group was random divided into two subgroups respectively, every 10 mice of one subgroup were injected subcutaneously with growth hormone （GH） （lmg/kg） daily from the 10th, 14th andl8th days respectively until the 22nd days, and the other subgroup received saline injection. Results All mice treated with GH have higher level of IGF-1, compared with those treated with saline. High level of IGF- 1 promoted the development of cachexia in these mice treated with GH from the 10th days. However, the level of IGF-1 has negative correlation with the cancer cachexia state for mice treated with GH from the 14th days. Conclusion Circulating IGF-1 and GH play an important role in tumor growth and cachexia development in the early stage of cancer and can ameliorate the state of cachexia in the advanced stage.