紫叶稠李叶片花色苷及其合成相关酶动态

Dynamic Changes of Anthocyanin and the Relevant Biosynthesis Enzymes in Padus virginiana 'Schubert' Leaves

以紫叶稠李为试材,以叶片为绿色的稠李为对照,测定叶片转色期2树种不同部位叶片中花色苷含量,以及花色苷合成相关酶苯丙氨酸解氨酶（PAL）、查儿酮异构酶（CHI）、二氢黄酮醇还原酶（DFR）和类黄酮糖基转移酶（UFGT）活性的变化。结果表明：5月到7月,紫叶稠李基部、中部、梢部叶片中花色苷的含量分别增加了219.40%、666.63%、76.81%。PAL活性与花色苷的含量呈显著的二次曲线关系,当PAL活性大于60U·g^-1FW时,花色苷的含量随着PAL活性的增加而增加;CHI活性与花色苷的含量呈极显著的线性关系,花色苷的含量随着CHI活性的升高而增加;当DFR活性为50-150U·g^-1FW,随着酶活性的升高花色苷的含量增加;UFGT活性与花色苷含量呈极显著的幂函数关系,其活性的提高能显著增加叶片中花色苷的含量。

Content of anthocyanin and the activities of enzymes in anthocyanin biosyntheses including plenylalanine ammonia lyase （PAL）, ehaleone ismoerase （CHI）, dihydroflavonol reduetase （DFR） and UDP glueose-flavonoid-3-o-glyeosyltranfe-rase （UFGT） were investigated using Padus virginiana ‘Schubert＇ in the leaf color-changing period, and P. resemosa was taken as control. The results indicated that from May to July the content of anthoeyanin in the leaves at the base, middle and top of branches in P. virginiana ‘Schubert＇ increased by 219.40 % , 666.63 % and 76.81% . The activity of PAL was quadratically correlated with the content of anthocyanin, and the content of anthocyanin increased as the PAL activity increased after it was higher than 60 U·g^-1FW. CHI activity was significantly and positively correlated with the content of anthocyanin. The content of anthocyanin increased also with an increase in DFR activity when it was between 50 and 150 U·g^-1 FW. There was a power function correlation between UFGT activity and content of anthocyanin, and the increase in UFGT activity could significantly raise the content of anthocyanin.