烟碱抗β-淀粉样蛋白神经毒性作用的研究

Nicotine against the neurotoxic effect of β-amyloid protein in the hippocampal cultures

目的探讨烟碱抗β-淀粉样蛋白(Aβ)神经毒性作用的机制。方法在海马神经组织细胞混合培养体系中分别加入烟碱、β-淀粉样蛋白,用MTT法、ELISA分别测定海马细胞增殖活性和培养细胞上清液中IL-1β含量。结果烟碱加Aβ组细胞增殖活性明显高于Aβ组。烟碱加Aβ组培养细胞上清液中IL-1β含量明显低于Aβ组。结论烟碱可抑制Aβ所致的IL-1β表达增加,对Aβ神经细胞毒性具有拮抗作用。

Objective To explore the mechanism of nicotine against the neurotoxic effect of β-amyloid protein in the hippocampal cultures, Methods To establish the mixed hippoeampal cultures,then the hippocampal cultures incubated with nicotine and β-amyloid protein,or with β-amyloid protein alone respectively. MTT and ELISA were adopted to estimate the cell viability and the levels of IL-1β of the culture supernatants. Results The cell viability of nicotine groups were higher than β-amyloid protein group. The levels of IL-1β in nicotine groups were decreased compared with the β-amyloid protein group. Conclusion Nicotine could inhibit the increase of IL-1β induced by Aβ protein,and against the neurotoxic effect of Aβ.