戊二醛结合肝素鞣制牛颈静脉带瓣管道抗钙化的实验研究

An experimental study of glutaraldehyde and heparin treatment on VBJVC

目的用动物皮下包埋的模型,用戊二醛鞣制的牛颈静脉带瓣管道(VBJVC)作为对照,来研究戊二醛结合肝素鞣制的牛颈静脉带瓣管道是否具有更好的抗钙化能力和抗衰老能力。方法把牛颈静脉带瓣管道分为两组,实验组(戊二醛结合肝素组)和对照组(戊二醛组),分别包埋在两组实验兔的皮下。90d后取出牛颈静脉带瓣管道片,进行钙的定量分析和形态学检查。形态学分析包括光镜下HE染色和Von-Kossa染色,进行钙化程度的比较。结果比较戊二醛结合肝素鞣制的VBJVC(A组)较之传统的单纯的用戊二醛鞣制的VBJVC(B组),其结果有明显的统计学意义。(1)标本用原子分光光度计测量组织钙含量,A组(13.92±5.03)mg/g,B组(34.99±12.33)mg/g,A组的组织钙化明显低于B组;(2)光镜下用Von-Kossa钙染色,按照Carpentier的分级方法,A组,0度2个,Ⅰ度3个,Ⅱ度1个。B组,0度1个,Ⅰ度1个,Ⅱ度2个,Ⅲ度2个,A组的组织钙化明显低于B组。结论经过戊二醛结合肝素鞣制的VBJVC,较之单纯用戊二醛鞣制的VBJVC,有较好的抗钙化能力。

Objective To find a better method of anticalcification of valved bovine jugular vein conduits（VBJVC）,we used a compound method of glutaraldehyde（GA） and heparin treatment on VBJVC,with animal subcutancous embeding experimental calcification model. Methods The 12 VBJVCs were divided into two groups：A group（GA and heparin groups） ,B group（GA groups）. In the animal experiment, 12 rabbits were randomly divided into 2 groups with 6 rabbits each group. The 12 VBJVCs were randomly embedded in the bodys of the 12 rabbits respectively,and then,the VBJVCs were taken out after 90 days. The calcium of the VBJVCs was measured with atomic absorption spectroscopy,the same time the VBJVCs were marked HE dye and Von Kossa dye. Resuits A group showed less calcification singnificantly as compared with B group after 90 days implantation[mean tissue calcium content （13.92±5.03）mg/g versus （34. 99±12.33）mg/g]. Pathohistologic examinations demonstrated most VBJVCs of the A group were only mild calcification. Conclusion VBJVC treated with glutaraldehyde and heparin appears to have less calcification.