芹菜素人体内代谢研究

Study on metabolism of apigenin from celery leaf in human

目的研究摄入芹菜叶后芹菜素在人体内的吸收、排泄。方法10名健康受试者，男女各半，年龄范围24～28岁，平均体质指数（BMI）为20．37kg／m^2。受试者按2g／（kg·bw）食用芹菜叶并食用不含芹菜素和毛地黄黄酮的食物。在分别摄入芹菜叶后4，6，7，8，9，10，11和28h，采集血样，并收集24h全尿样。用高效液相色谱-电化学检测器（HPLC—ECD）检测血浆、红细胞和尿中芹菜素含量。结果摄入芹菜叶后．全部受试者血浆中芹菜素浓度呈上升趋势，（7．70±0．82）h血浆芹菜素浓度达最高水平（207．11±97．55）nmol／L．在28h内下降到检出限（2．32nmol／L）以下；受试者尿样中芹菜素平均含量为（2251．72±1304．22）nmol／L24h，相当于摄入量的（0．62±0．37）％；红细胞中芹菜索含量在备时间点差异无统计学意义（P〉0．05）。结论通过食物摄入到体内的芹菜素一部分进入了人体循环系统．可以在体内发挥生物学作用。

Objective Absorption and excretion of apigenin was tested after the ingestion of celery leaf. Methods Ten healthy subjects（5 women, 5 men） in the age range of 24--28 years and with an average body mass index of 20.37 kg/m^2 took part in this study. After an apigenin - and luteolin - free diet, a single oral celery leaf of 2 g/（kg·bw） was consumed. Blood samples were taken at 4, 6, 7, 8, 9, 10, 11 and 28 h after celery leaf consumption and 24h urine samples were collected. Apigenin was analyzed in plasma, urine and red blood cells by means of HPLC - ECD, Results A maximum apigenin plasma concentration of （207.11 ± 97.55 ） nmol/L was reached after （ 7.70 ± 0.82） h, For all participants, plasma apigenin concentration rose after celery leaf ingestion and fell within 28h under the detection limit（2.32 nmol/L）, The average apigenin content in urine was（2251.72 ± 1304.22 ）nmol/L 24h corresponding to（0.62± 0.37） % of the ingested dose, The apigenin could be detected in red blood cells without time - response characteristics, Conclusion A small portion of apigenin provided by food reaches the human circulation and, therefore, may reveal biological effects.