黄芪、党参提取物增强紫杉醇抑制肿瘤血管生成和转移的实验研究

Inhibitory effect of huangqi and dangshen extraction with pacilitaxel on metastasis and angiogenesis on mouse Lewis lung carcinoma model

目的:观察黄芪、党参提取物对紫杉醇抑制Lewis肺癌血管生成和肿瘤转移的增强作用,为中医药抗肿瘤应用提供实验依据。方法:C57BL/6小鼠右肋处皮下接种Lewis肺癌,6h后随机分为实验组、紫杉醇组和对照组。实验组接受紫杉醇联合参芪注射液治疗,紫杉醇组接受同等剂量的紫杉醇治疗,对照组用等体积的生理盐水代替。观察指标:移植瘤体积,肿瘤组织内微血管度和肺脏转移瘤数量,小鼠存活时间。结果:与紫杉醇治疗相比,紫杉醇联合参芪可以明显减少移植瘤内的微血管密度(10.1±4.4 vs 16.8±7.3,P<0.05)和肺脏转移瘤个数(13.4±4.3 vs 18.4±3.9,P<0.05),明显延长小鼠的存活时间(43.3±8.4d vs 36.3±6.6d,P<0.05)。结论:黄芪、党参提取物对紫杉醇抑制肿瘤血管生成和移植肿瘤转移有一定的增强作用。

AIM： To study enhance effect of huangqi and dangshen extraction （Shenqi） on pacilitaxel inhibitory metastasis and angiogenesis on mouse Lewis lung carcinoma model, METHODS： Lewis lung carcinoma cells were inoculated into right hind footpad of CS？BL/6 mice, Six hour after tumor inoculated, the mice were randomly divided into 3 groups, Shenqi （ paclitaxel plus Shenqi） or paclitaxel was intraperitoneally injected in two group since the second day of the establishment of animal model, The third group simply administered with normal saline was set as placebo-control. Tumor volume, quantitation of microvessel density （MVD） in inoculated tumor, the number of metastasis in the lungs and survival analysis were compared in 3 groups. RESULTS： Paclitaxel plus Shenqi can effectively reduced MVD in inoculated tumor and the number of lung metastasis as compared with other two group （ P 〈 0.05 ）. The survival time of Shenqi group was also significantly longer （ P 〈 0.05）. Tumor volume was no statistical difference in three group （P〉0.05）. CONCLUSION： Shenqi can amplify the paclitaxel effect of anti-angiogenesis and anti-metastasis, enhances the survival time of mice bearing LLC, might has possible therapeutic applications in the treatment of lung cancer.