接种根瘤菌的台湾相思对干旱胁迫的生化响应

Biochemical response of drought stressed Acacia richii seedlings inoculated with rhizobia

运用PEG-6000高渗溶液模拟干旱胁迫实验,测定台湾相思Acacia richii接种不同根瘤菌后体内部分生化物质的变化趋势,并探讨接种不同根瘤菌后对体内游离脯氨酸、可溶性糖和叶绿素积累的影响,从而为筛选更有利于台湾相思在干旱环境下生存的根瘤菌提供支持。结果表明,在一定时间内,接种不同根瘤菌均能提高代谢物质的累积,但从不同寄主分离得到的根瘤菌其作用有一定的差异。在分离自白灰毛豆Tephrosia candida(菌株SMD)、银合欢Leucaena leucocephala(菌株YHH)、台湾相思(菌株TWXS)的根瘤菌中,接种SMD更能促进台湾相思体内游离脯氨酸等代谢物质的累积。代谢物质的累积是台湾相思接种根瘤菌后对干旱胁迫的适应性表现,来自白灰毛豆的根瘤菌对促进相思苗木在干旱环境中的抗逆性具有更大的潜力。

To determine if in a drought environment there was an adaptation for Acacia richii due to rhizobia, this experiment analyzed the accumulation of metabolites, such as proline, soluble sugar, and chlorophyll, after inoculation with SMD rhizobia separated from Tephrosia candida, YHH from Leucaena leucocephala and TWXS from A. richii under artificial drought conditions in a PEG-6000 solution. The contents of PEG-6000 were 0 （control） , 0. 010, 0. 025 and 0. 037 respectively. Results showed that different rhizobia increased metabolite accumulation in drought conditions, but there were different levels of accumulation depending on rhizobia strains. The rhizobia SMD had a stronger potential to promote seedling resistance in a drought environment than rhizobia YHH and TWXS. Thus, it is advantageous to choose rhizobia to promote growth of A. richii in drought conditions. [ Ch, 3 tab. 16 ref. ]