海洋蛋白肽对小鼠免疫调节作用的实验研究

The study on the immunomodulating effect of marine protein peptides in mice

目的探讨海洋蛋白肽（marine protein peptide，MPP）对小鼠的免疫调节作用及其可能的机制。方法以小鼠为动物模型，观察不同剂量（0．22g／kg、0．45g／kg和1．35g／kg）MPP对小鼠体重、免疫器官相对重量、细胞免疫功能、体液免疫功能、单核巨噬细胞功能、NK细胞活性、脾脏T淋巴细胞亚群以及血清中细胞因子的影响。结果MPP0．22g／kg剂量组ConA诱导的小鼠淋巴细胞增殖能力（0．33±0．21）和足跖肿胀度（0．36±0．11）mm，较对照组的相应两值（0．15±0．10）和（0．21±0．10）mm均有显著提高。溶血空斑数对数转换值在MPP0．22g／kg组（1．64±0．06）、0．45g／kg组（1．59±0．05）和1．35g／kg组（1．56±0．10）中均显著高于对照组（1．38±0．10）；血清半数溶血值在MPP0，22g／kg（141．00±23．00）和0．45g／kg组（130．40±33．20）中均显著高于对照组（100．30±19．40）。MPP0．22g／kg组的NK细胞活性对数转换值（1．672±0．142）比对照组（1．392±0．182）明显升高。CD4’T辅助细胞（Th细胞）百分比在MPP0．22g／kg组（32．84±3．776）％和0．45g／kg组（32．42±3．507）％中均显著高于对照组（25．06±0．354）％。血清IL一2浓度在MPP0．22g／kg组181．06pg／ml、0．45g／kg组94．84pg／ml和1．35g／kg102．61pg／ml中均显著高于对照组0．50pg／ml；血清IL-5浓度在MPP0．22g／kg组38．31pg／ml中显著高于对照组0．50pg／ml。结论MPP具有增强小鼠免疫功能的作用，可能通过增强Th细胞功能以及刺激细胞因子分泌而实现。

Objective To observe the effects of marine protein peptide （MPP） on immunnomodulating in mice and its possible mechanism. Methods Female ICR mice （6-8 weeks old） were administered the MPP for 4 weeks with the dose of 0. 22 g/kgBW 、0.45 g/kgBW and 1.35 g/kgBW. Spleen and thymus were weighted and cell - mediated immune functions, humoral immune functions, phagocytic functions of mononuclear phagocyte, NK cell activity assays, the T cell subpopulation of the spleen tissue by the flow cytometer and the concentrations of cytokines in serum by cytometric bead array were examined. Results The capacity of lymphocyte proliferation induced by Con A （0. 33 ± 0. 21 ）, DTH response （ 0. 36 ± 0. 11 ）mm in MPP 0. 22 g/kgBW group were significantly increased in comparison with these values in control group （0. 15 ±0. 10） and （0. 21 ±0. 10）ram, respectively, P 〈0. 05. IgM - PFC number of MPP 0. 22g/kgBW group （ 1.64 ± 0. 06） ±0. 45 g/kgBW group （ 1.59 ± 0. 05 ） and 1.35 g/kgBW group （ 1.56 ± 0. 10） were higher than those in control group （ 1.38 ± 0. 10 ）, P 〈 0. 01 ; and the level of serum HCs0 of MPP 0. 22g/kgBW group （ 141.00 ± 23.00 ） and 0.45 g/kgBW group （ 130.40 ± 33. 20 ） were greater than the control （ 100. 30 ± 19.40）, P 〈 0. 01. The activity of NK cells in MPP 0. 22 g/kgBW group （1. 672 ±0. 142） was significantly elevated in comparison with this value in control group （1. 392 ± 0. 182）, P 〈 0. 05. The percentoge of CD4 ± T helper （Th） cell in spleen of MPP 0. 22g/kgBW group （ 32. 84 ± 3. 776） % and 0. 45 g/kgBW group （ 32. 42 ± 3. 507 ） % was higher than those in control group （25.06 ±0. 354） %, P 〈0. 05. The concentrations of IL-2 in serum of MPP 0. 22g/kgBW group 181.06 pg/ml,0. 45 g/kgBW group 94. 84 pg/ml and 1.35 g/kgBW group 102. 61 pg/ml were higher than those in control group 0. 50 pg/ml, P 〈0. 05 ; and the level of IL-5 of MPP 0. 22g/kgBW group （38. 31 ） pg/ml was greater than the control 0.50 pg/ml, P 〈 0. 05. Nevertheless, no obvious effects on weight increasing, the ratio of immune organ and body weight and phagocytosis capacity were observed in our study. Conclusion MPP could improve the immune functions in mice, and might be by the mechanism of enhancing the function of Th cells stimulating the secretion of Thl and Th2 type cell cytokines.