二甲亚砜、吐温80对小鼠骨髓来源细胞体外生长和活力影响的量效关系分析

Dose-Effect Relationship of DMSO and Tween 80 Influencing the Growth and Viability of Murine Bone Marrow-Derived Cells in Vitro

本研究观察二甲基亚砜、吐温80对小鼠骨髓基质细胞(BMSC)、粒-巨噬系祖细胞(CFU-GM)和内皮细胞系细胞(BMEC)体外生长和活力的影响,并分析其量效关系。分别设置不同终体积分数二甲亚砜与吐温80的细胞培养体系及其对照,检测成纤维细胞形成单位(CFU-F)和CFU-GM的集落生成率,用MTT比色法和台盼蓝排斥试验测定BMSC和BMEC的细胞活力和活细胞百分率。结果表明:二甲亚砜为2%、吐温80为0.005%-0.01%时,CFU-F、CFU-GM集落产率与对照组相比显著降低(p<0.05或<0.01)。二甲亚砜为0.5%-1.0%、吐温80为0.002%-0.005%时,BMSC和BMEC的细胞活力和活细胞率与对照组相比显著减低(p<0.05或<0.01)。随着二甲亚砜、吐温80在上述体积分数上增加,各相应检测指标的减低率递增。结论:二甲亚砜,吐温80在细胞培养体系中达一定含量时,对小鼠骨髓BMSC、CFU-GM和BMEC细胞具有明显的生长抑制作用和(或)细胞毒性作用,含量增高则效应更显著。

This study was purprosed to examine the effect of dimethyl sulfoxide （DMSO） and Tween 80 on the growth and viability of stromal cells （BMSC）, colony-forming units for granulocytes and macrophages （CFU-GM） and bone marrow endothelial cell line （BMEC） from murine bone marrow in vitro, and to analyze the concentration-effect relationship. The colony yields of colony-forming units fibroblastic （CFU-F） and CFU-GM were assessed in the murine bone marrow cell cultures at various concentrations of DMSO or Tween 80 and in the control groups. The MTT assay and trypan blue exclusion were used to determine the cell viability and percentage of survival in BMSC and BMEC cultures with or without either of these organic solvents. The results showed that the colony yields of both CFU-F and CFU-GM were decreased significantly （p 〈 0.05 or 〈 0.01 ） at the concentrations （ v/v final） of 2% DMSO or 0.005% - 0.01% Tween 80 respectively, as compared with control. The cell viability and percentage of survival of BMSC and BMEC cultures were significantly reduced （p 〈 0.05 or 〈 0.01 ） at 0.5% - 1.0% DMSO or 0.002% - 0.005% Tween 80, as compared with control. With the increase of volume fractions of these solvents, the decreased percentages of corresponding measurements were increased by degrees. It is concluded that when the concentration of DMSO or Tween 80 goes to a certain level in cell culture medium, either of the organic solvents has an inhibitory action or/and cytotoxicity on the growth and viability of BMSCs, CFU-GM and BMECs. The growth inhibition and cytotoxic response are more significant at higher concentrations of these solvents.