沙眼衣原体感染细胞抗凋亡及Bim的表达

Study on Resistance to Apoptosis and the Expression of Bim in Chlamydia-infected Cells

目的:研究沙眼衣原体(D血清型)感染的Hela229细胞Bim蛋白质的表达及抵抗凋亡的情况。方法:Western-blot检测沙眼衣原体感染和未感染的Hela229细胞Bim蛋白质的表达水平。凋亡诱导剂etoposide作用Hela229细胞后,琼脂糖凝胶电泳检测DNA Ladder带;流式细胞仪检测凋亡率。结果:Hela229细胞在未感染沙眼衣原体时可检测到Bim的表达;在感染24小时、48小时后均未检测到Bim的表达。经etoposide作用后,未感染的Hela229细胞检测到DNALadder带;流式细胞仪检测的凋亡率为90.64%。感染24小时的Hela229细胞,未检测到DNA Ladder带;凋亡率为11.50%,与未感染的Hela229细胞诱导后的凋亡率比较有统计学意义(P<0.05)。结论:沙眼衣原体感染的Hela229细胞Bim蛋白质的表达下降;并能抵抗etoposide诱导的细胞凋亡。

Objective： To explore the expression of Bim in Chlamydia traehomatis - infected Hela229 cells and resisting apopto- sis. Methods： Western - blot was applied to detect the expression of Bim at protein level in C. traehomatis - infected and uninfected Hela229 cell. Induced by etoposide, Detection of cell apoptosis： DNA Ladder fragment were detected by agarose gel eleetrophoresis, Apoptotie index were analyzed by flow eytometer assay （ FCM ）. Results： Western - blot showed that Bim was detected in uninfected C. traehomatis Hela229 cell. But Bim was not detected in Hela229 cell infected C. traehomatis after 24 hours and 48 hours. Induced by etoposide, uninfected Hela229 cells were detected DNA ladder eleetrophoresis fragmems. FCM showed that the apoptotie rate was 90. 64% in uninfected Hela229 cells. While Hela229 cells infected 24 hours with C. traehomatis was induced by etoposide, there were no DNA ladder eleetrophoresis fragments. Its apoptotie rate was 11.50%. There were significant difference compare with uninfected Hela229 cells induced by etoposide（ P 〈 0.05 ）. Conclusions： The expression of Bim of C. traehomatis -infected Hela229 cells was reduced. C. traehomatis infection can resist apoptosis induced by etoposide.