小鼠肝脏内组成型雄烷受体基因表达剪切突变体的研究

Alternatively spliced variants of constitutive androstane receptor in liver of mouse

目的:研究组成型雄烷受体(CAR)在小鼠肝脏内是否存在剪切突变体。方法:以小鼠的肝脏为原料提取核酸,采用逆转录的方法获得小鼠的组成型雄烷受体的cDNA序列,并将其构建到T载体中进行测序分析。结果:实验发现CAR在小鼠肝脏内有不同的剪切体存在。结论:剪切体的不同主要集中在受体结合部位,估计这种不同与不同外源性物质对受体的调节功能不同有关。

Objective： To investigate the existence of alternatively spliced variants of constitutive androstane receptor （CAR） in liver of mouse. Methods： The nucleotide from liver of mouse was purified and the CAR cDNA was amplified by PCR. The fragments of CAR cDNA were cloned to T vector and sequence analysis was performed. Results： Various spliced variants of CAR in liver mouse were confirmed by DNA sequencing. Conclusion： There are alternatively spliced variants in CAR,which are located in the ligand binding sequence of CAR.