鸡肝散药效成分木犀草素在大鼠体内的排泄研究

Luteolin excretion after oral administration of Elsholtzia blanda benth extracts in rats

目的：建立RP—HPLC法测定大鼠尿、粪便、胆汁中鸡肝散药效成分——木犀草素的含量，研究木犀草素在SD大鼠中的排泄情况。方法：给SD大鼠灌胃鸡肝散提取物后，于不同时间段采集尿样、粪便、胆汁，经处理后，以苷元木犀草素为目标物，分别测定其在不同时间段尿样、粪便、胆汁中的浓度。结果：尿样、粪便、胆汁中木犀草素的方法回收率为98．00％～106．00％，提取回收率为85．00％～108．00％，日内及日间精密度均小于10．00％。木犀草素的总累积排泄率约为37％（测得尿中约11％；粪便和胆汁中各约26％）。结论：建立的大鼠尿、粪便、胆汁中木犀草素的含量测定方法具有灵敏、准确，专属性强等特点。

Objective： To observe the excretion of luteolin after oral administration of Elsholtzia blanda benth extracts in rats. Methods： Samples of urine,feces and bile were collected after oral administration of Elsholtzia blanda benth extracts in rate. After deconjugation with β-glucuronidase/sulfatase, the levels of luteolin in urine, feces and bile were measured by RP -HPLC. Results： The recovery rate of luteolin was 98. 0%-106. 0% and the extract recoveries were 85.0%-108.0%. Relative＇standard deviation （RSD） of intra- and inter-day assay was less than 10.0%. The total accumulative excretion was 37% （11% in urine,26% in feces and bile）. Conclusion： The established RP-HPLC method is sensitive ,specific ,accurate ,and is applicable for determination of luteolin in rat urine,feces and bile.