葡萄糖转运蛋白在非小细胞肺癌中的表达及其临床意义

Expression of glucose transporter in non-small cell lung carcinoma and its clinical significance

目的:探讨葡萄糖转运蛋白(Glucose transporter,Glut)的两种异构体Glut1、Glut3及缺氧诱导因子-1(Hypoxia inducible factor-1,HIF-1)的亚基HIF-1α,在非小细胞肺癌中的表达及临床意义。方法:选取34例手术切除非小细胞肺癌(包括癌组织和癌旁组织)和17例肺良性病变标本,用免疫组织化学法测定Glut1、Glut3及HIF-1α在肺组织中的表达;用RT-PCR半定量检测Glut1、Glut3及HIF-1α的mRNA表达;用Western Blot检测Glut1、Glut3及HIF-1α蛋白的表达,并测两者相关性。结果:Glut1、Glut3及HIF-1α在肺癌组织中mRNA相对含量为0.689±0.245、0.506±0.246、0.693±0.248,对应癌旁组织为0.338±0.157、0.482±0.238、0.351±0.184,Glut1和HIF-1α在肺癌组织中显著升高(P<0.001),而Glut3差别无显著性(P>0.05)。Glut1、Glut3、HIF-1α在癌组织中蛋白相对含量为0.582±0.247、0.551±0.251、0.525±0.246,癌旁组织为0.288±0.151、0.436±0.224、0.261±0.135,在肺良性病变中为0.291±0.142、0.402±0.206、0.271±0.176,Glut1和HIF-1α在癌组织中的表达均明显高于癌旁组织(P<0.001)和肺良性病变组织(P<0.001);而Glut3差别无显著性(P>0.05)。Glut1和HIF-1α在低分化组明显高于中高分化组(P<0.05),期明显高于和期(P<0.05),且HIF-1α的表达与Glut1明显相关(r=0.854,P<0.01)。结论:在非小细胞肺癌中,Glut1和HIF-1α存在高表达,其表达与细胞分化程度、TNM分期密切相关,且Glut1和HIF-1α具有相关性。

Objective： To investigate the expression of glucose transporter （Glut） 1,Glut3,and hypoxia inducible factor （HIF）-1α in human non-small cell lung carcinoma （NSCLC）,and its clinical significance. Methods： Specimens of cancer tissues and paracancerous lung tissues from 34 cases of NSCLC and 17 specimens of benign lung lesions were collected. The expressions of Glutl, Glut3, and HIF-1α were detected with immunohistochemical staining, RT-PCR, and Western blot. Results： The relative mRNA expressions of Glut l and HIF-1α were 0. 689±0. 245, 0. 693α0. 248 in cancer tissues; and 0. 338±0. 157,0. 351±0. 184 in paracancerous lung tissues （P〈0. 001）; while those of Glut3 were 0. 506±0. 246 in cancer tissues and 0. 482±0. 238 in paracancerous tissues （P〉0.05）. The relative protein expressions of Glut1 and HIF-1α were 0. 582±0. 247,0. 525±0. 246 in cancer tissues and 0. 288±0. 151,0. 261±0. 135 in paracancerous lung tissues （P（0. 001）,but the protein expressions of Glut3 were 0. 551±0. 251 and 0. 436±0. 224 respectively （P〉0.05）. Glut1 and HIF-1α expressions were higher in poor differentiation group and in stage Ⅲ group,than those in medium and well differentiation group and stage Ⅰ and Ⅱ group. Moreover,there was a significant correlation between the expression of Glut1 and HIF -1α（r=0. 854,P〈0.01）. Conclusions： Glut1 and HIF-1α are highly expressed in NSCLC,and their expressions are associated with tumor differentiation and clinical stage.