摘要
利用ＰＥＧ诱导原生质体融合技术，进行糖化酵母单倍体２６００７（α，ＳＴＡ，ｉｎｈ，ａｄｅ）和去除了ＩＮＨ１基因的耐高温酒精酵母单倍体菌株Ｚ６－１０（α，ＳＴＡ，ｉｎｈ，ａｒｇ）的同型原生质体融合实验，在再生基本培养基上挑选融合子，获得一株既具有较强糊精利用能力，又耐高温和个产酒精的融合株Ｆ４１。经与亲本酒精酵母二倍体菌株２１００２５玉米发酵性比较，使用Ｆ４１菌发酵可减少４０％的糖化酶用量。
The aim of this work was to reduce the amount of enzyme used in saccharification by using good fermenting amylolytic yeast strains. By protoplast fusion of identical mating type haploids, fusants were selected as regenerated coonies on a hypertonic minimal medium at 35℃. It was found that the added glucoamylase could be reduced by over 40% using fusant F41 strain as compared to the parental distiller's yeast.
出处
《天津轻工业学院学报》
1997年第1期1-7,共7页
Journal of Tianjin University of Light Industry
关键词
糖化酵母
酒精酵母
原生质体融合
乙醇
saccharomyces diastaticus
distiller's yeast
protoplast fusion
