沙立度胺抑制乳腺癌细胞增殖的体外实验

Inhibitory Effects of Thalidomide on the Proliferation of Breast Cancer Cells

背景与目的:观察沙立度胺对体外乳腺癌细胞MCF_7和MDA_MB_231的抑制作用,以及对细胞凋亡和细胞周期的影响。材料与方法:经不同浓度(10μg/ml、50μg/ml、100μg/ml、200μg/ml)的沙立度胺处理MCF_7和MDA_MB_231细胞不同时间(24h、48h、72h)后,采用四甲基偶氮唑蓝(MTT)法检测各组细胞存活和生长情况,分析量效关系、时效关系。采用流式细胞仪检测沙立度胺对细胞凋亡和细胞周期的影响。结果:在10～200μg/ml浓度范围,沙立度胺对MCF_7和MDA_MB_231细胞增殖均有抑制作用(P<0.01)。在药物作用48h后,对各组细胞的抑制率均达到高峰,尤以100μg/ml浓度组抑制率最高。沙立度胺作用后的乳腺癌细胞在流式细胞分析图上见细胞凋亡峰(亚G1峰),100μg/ml沙立度胺作用48h后,MCF_7和MDA_MB_231细胞凋亡率分别为9.66%和11.18%,与对照组凋之率比较差异有统计学意义(P<0.01)。结论:沙立度胺对MCF_7和MDA_MB_231细胞的体外增殖具有明确抑制作用,并可诱导细胞凋亡。

BACKGROUND AND AIM To investigate the effects of thahdomide on the proliferation in breast cancer cell lines. MATERIALS AND METHODS： Establishing the solvent control against and different densities, different durations, in order to study the relation between time and efficacy, The growth rate among these groups was measured by MTT assay. The influence of thalidomide on apoptosis RESULTS： The growth rates in these groups were different, thalidomide could inhibit the proliferation of MCF-7 and MDA-MB-231 from 10 μg/ml to 200 μg/ml. The peak inhibitory effect occurred after thalidomide treatment for 48 h. 100 μg/ml had the maximal inhibitory effect after 48 h, Apoptosis was found in the breast cancer cells in the second period of Gi in the DNA histogram of FCM. 100 μg/ml thalidomide treatment for 48 h, the MCF-7 cell apoptotic index could amount to 9.66% and MDA-MB-231 cell to 11.18%, comparing with control groups, the difference was significant（P 〈0.01） CONCLUSION Thalidomide could inhibit the proliferation and induce apoptosis of breast cancer cell lines MCF-7 and MDA-MB-231.