摘要
背景与目的:探讨血管内皮生长因子_C(vascular endothelial growth factor_C,VEGF_C)和环氧合酶_2(cyclooxyenase_2,COX_2)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及其与淋巴管生成、淋巴结转移的关系。材料与方法:采用免疫组化SP法检测60例OSCC、23例口腔黏膜癌前病变、19例其它口腔黏膜良性病变组织中COX_2、VEGF_C及VEGFR_3受体的表达,计数肿瘤组织中淋巴管密度(lymphatic vessels density,LVD),结合临床病理因素进行分析。结果:OSCC中VEGF_C、LVD和COX_2表达明显高于口腔癌前病变和其它良性病变。OSCC中VEGF_C蛋白表达、LVD与淋巴结转移呈明显正相关关系(P<0.05);COX_2蛋白表达与肿瘤的淋巴结转移、临床分期明显相关(P<0.05)。VEGF_C和COX_2的表达呈正相关(r=0.519,P<0.01),两者表达与LVD有正相关关系(r=0.661,P<0.01;r=0.485,P<0.01)。结论:OSCC中VEGF_C和COX_2高表达,COX_2可能参与VEGF_C淋巴管生成通路,在肿瘤淋巴转移中发挥重要作用。
BACKGROUND AND AIM： To investigate the expression of VEGF-C, VEGFR-3 and COX-2 in oral squamous cell carcinoma（OSCC）, and the correlation with lymphangiogenesis and lymph node metastasis. MATERIALS AND METHODS： Immunohistochemical SP method for VEGF-C, VEGFR-3 and COX-2 were performed in 60 cases of OSCC, 23 cases of precancerous change and 19 benign lesion specimens. The lymphatic vessel density（LVD） in the tumor was counted and analyzed with clinicopathologic parameters. RESULTS： Compared with benign and precancerous change, the expressions of VEGF-C, LVD and COX-2 were significantly higher in OSCC （P 〈 0.05） . The expressions of VEGF-C and LVD were significantly correlated with lymphatic metastasis （P 〈 0.05）; COX-2 was obviously related with clinical stage and lymphatic metastasis（P 〈 0.05） . The expression of COX-2 was closely correlated with that of VEGF-C（r = 0,519, P〈0.01）, with LVD positively correlated with their expression levels（r = 0.661, P〈0.01 and r = 0.485, P 〈 0.01, respectively） . CONCLUSION： The expression rates of COX-2 and VEGF-C were high in OSCC. COX-2 may participate in VEGF-C lymphangiogenic pathway and play an important role in the lymph node metastasis of OSCC.
出处
《癌变．畸变．突变》
CAS
CSCD
2008年第2期103-106,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
安徽省教育厅自然科学基金资助项目(2005kj379)
