用染色体涂染方法对早先辐射受照人员进行回顾性照射剂量估算

Dose Reconstruction for Persons with Previous Accidental Irradiation Exposure by Chromosome Painting Method

背景与目的:探索用染色体涂染(painting)技术回顾性估算早先辐射受照人员照射剂量的可行性。材料与方法:用不同剂量(0～5.00Gy)的60Coγ射线照射正常人外周血,用1、2和4号染色体涂染探针分析染色体易位,建立辐射诱发的染色体易位率剂量_效应曲线。用同样的方法分析3例早先受60Coγ射线照射人员的染色体易位,参照剂量_效应曲线估算剂量,与照射后当时估算的生物剂量比较;并对1例无照射当时的生物剂量病例进行回顾性剂量估算。结果:用painting方法分析0～5.00Gy60Coγ射线诱发的全基因组易位率均随着吸收剂量的增加而增高,吸收剂量和全基因组易位率之间的剂量_效应曲线均为二次方程模式,曲线方程为^Y=0.043D2+0.006D+0.0036。3例早先受照人员的估算剂量与照射后生物剂量的资料基本一致,另1例早先受照人员的估算剂量与照后当时物理模拟剂量一致。结论:本研究建立的painting方法可用于早先受辐射照射人员的回顾性剂量估算。

BACKGROUND AND AIM： To investigate the applicability of painting method in retrospective dose estimation. MATERIALS AND METHODS： Healthy human peripheral blood samples were irradiated with 0- 5.00 Gy ^60Co γ-rays. Then the chromosome translocations in these samples were detected with painting method using chromosomes 1, 2 and 4 probes. The dose-response curve of absorbed dose and genome translocation frequency were established. The retrospective doses were estimated for 3 individuals, who previously had accidental radiation exposure and had biodosimetry data shortly after the exposure. And similar estimation was done for another individual without biodosimetry data but with the physical dose data. RESULTS： The genome translocation rates induced by 0- 5.00 Gy ^60 Co γ-rays detected by painting were increased with the absorbed dose. The dose-response curve was Y = 0.043D^2＋ 0.006D ＋ 0. 0036 for painting.The retrospective doses for the 4 individuals were similar with the previous biodosimetry data or the physical dose data. CONCLUSION： Dose reconstruction for previous radiation exposure individuals could be carried out with the painting method established in this study.