抗金黄地鼠精液抗体作为杀精子剂的实验研究

Experimental Research on Anti-semen Antibody of Golden Hamster as Spermicide

背景与目的:探讨抗精液抗体作为阴道内杀精子剂的可行性。材料与方法:制备抗金黄地鼠精液抗血清,用抗血清与金黄地鼠精液混合后,做体外精子活率分析、精子凝集实验;用流式细胞仪分析精子线粒体功能,取雌鼠卵细胞做体外受精及精卵融合试验;并做抗血清处理后精子的子宫内注射实验和交配后阴道内精子活率测定,以探讨抗精液抗体在体外和体内制动精子的作用。结果:抗精液抗体能够完全凝集和制动精子,流式细胞仪检测结果显示抗血清处理组精子线粒体荧光强度为180.28±82.24,明显低于对照组(309.74±148.37),差异具有统计学意义(P<0.01);体外受精实验中处理组的受精率为0.9%,显著低于对照组的37.50%(P<0.01);子宫内精子注射实验中实验组受精卵率为1%,显著低于对照组的15%(P<0.01);交配后阴道内精子活率测定显示,实验组中所有精子均不活动,而对照组精子活率为60%。结论:抗精液抗体在体外、阴道和子宫内都能够完全凝集和制动精子,可以阻止精卵融合。抗精液抗体可以作为阴道内杀精子剂。

BACKGROUNO AND AIM： To study the feasibility of anti-semen antibody as spennicide. MATERIALS AND METHODS： Anti-semen antibody of golden hamster was prepared to incorporate with golden hamster semen, then sperm motility rate analysis, spermagglutination test, sperm mitochondrion function analysis, in vitro fertilization, intrauterine sperm injection and post mate endovaginal sperm motility analysis were performed to assess the inhibitory effect of anti-semen antibody on sperm in vitro and in vivo. RESULTS： Anti-semen antibody could agglutinate and immobilize sperm completely. In experiment group and control group, the average of sperm mitochondrion fluorescence intensity was 180.28 ± 82.24 and 309.74 ±148.37, the rate of in vitro fertilization was 0.9% and 37.50%, the rate of fertilization in intrauterine sperm injection test was 1% and 15.0%, the sperm motility rate in post mate endovaginal sperm motility analysis was 0 and 60.0%, respectively.All the differences between the experiment group and the control group were statisti cal significant （P 〈0.01）. CONCLUSION： Anti-semen antibody could agglutinate and immobilize sperm in vitro, in uterine and in vagina, and it could be used as a potential spennicide.