弓形虫感染与精子凋亡

Infection of Toxoplasmosis and apoptosis of spermatozoa

目的探讨弓形虫(Toxoplasmosis)感染与精子凋亡的关系。方法采用瑞-姬氏染色和末端脱氧核苷酸转移酶(TdT)介导的脱氧核苷酸原位末端标记法(TUNEL),对精浆抗弓形虫抗体(ATAb)检测阳性及阴性的男性精子进行凋亡率测定。结果弓形虫感染组中精子凋亡率明显高于弓形虫阴性组(P<0.05)。结论弓形虫感染可引起精子凋亡率升高,影响精子活率,为阐明弓形虫感染引起男性不育的机制提供了客观依据。

Objective：To evaluate the relationship between Toxoplasmosis infection and sperm apoptosis.Methods： Apoptotic spermatozoa were examined by Wright Giemasa staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate（dUTP）-biotin nick-end labeling（TUNEL） technique.To detect the apoptosis rate of spermatozoa of which culture for anti-toxoplasma antibody（ATAb）infection was positive or negative.Results： The rate of apoptotic spermatozoa wase significantly higher in the positive group of Toxoplasmosis infection than in the negative group（P〈0.05）.Conclusion：Toxoplasmosis infection may bring about elevation of apoptotic rate of spermatozoa and affect sperm motility,and it provides objective evidences for illustrating（explaining） the mechanism that cause male infertility.