三氧化二砷对人脐静脉内皮细胞作用的研究

Antiangiogenesis effect of arsenic trioxide on human umbilical vein endothelial cell

目的以人脐静脉内皮细胞作为血管新生内皮细胞的模拟物,通过研究As2O3对人脐静脉内皮细胞的影响,探讨As2O3的抗血管新生作用机制。方法分离和培养人脐静脉内皮细胞,以不同浓度和时间的As2O3和VEGF作用于脐静脉内皮细胞,应用流式细胞技术测定As2O3和VEGF对脐静脉内皮细胞增殖、细胞凋亡和癌基因bcl-2表达的影响。结果As2O3以时间和剂量依赖的方式抑制内皮细胞增殖,促进细胞凋亡,抑制癌基因bcl-2表达,而VEGF可拮抗As2O3的部分作用。结论As2O3可以通过直接作用于内皮细胞而抑制血管新生。

Objective To investigate the antiangiogenesis effect of arsenic trioxide on human umbilical vein endothelial cell. Methods Using flow cytometry to check the effect of As2O3 on the proliferation, apoptosis, expression of bcl-2 in cultured human umbilical vein endothelial cells. Results As2O3 could decrease proliferation, expression of bcl-2, and induce apoptosis of HUVEC in dosage and time dependent manners. Conclution As2O3 Could act antiangiogenesis effect on human umbilical vein endothelial cells.