应用胶体金试纸条诊断方法检测牛结核病的试验

Application of Colloidal Gold Testing Paper Method in Diagnosis of Bovine Tuberculosis

采用牛结核MPB70/83抗体检测胶体金试验方法对贵阳周边及某县奶牛场共309头奶牛进行牛结核病检测,同时采用结核菌素（PPD）皮试进行对比试验,结果显示,该病的感染率为4.48%-6.47%,胶体金试纸条试验与PPD反应具有一定的符合率[75%（15/20）],采用生物统计分析,进行χ2检验,计算得χ2c=0.703,χc2〈χ20.05,显示两种试验差异极不显著。同时试验还证明了牛结核MPB70/83抗体检测胶体金试纸条试验方法具有快速、简便、特异、敏感的特点,并且可作为PPD反应的辅助诊断方法。因此,该方法具有良好的应用前景。

309 cows in Guiyang dairies were tested by the colloidal gold test paper and PPD methods to diagnose bovine tuberculosis to compare two methods at the same time. The results show that the infection rate of bovine tuberculosis is 4. 48 % - 6. 47 % and there is no significant difference in diagnosis of bovine tuberculosis between two methods. The colloidal gold test paper method with advantages of rapidity, simplicity, speciality and sensitivity can be widely used as the supplementary diagnosis method for PPD method in future.