超声激活血卟啉声动力作用对鸡胚绒毛尿囊膜VEGF表达的影响

Effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in chick chorioallantoic membrane

目的:本研究探讨超声激活血卟啉(Hematoporphrin,Hp)声动力作用对鸡胚绒毛尿囊膜(Chorioallantoie membrane,CAM)的血管内皮细胞生长因子(Vascular endothelial cell growth factor,VEGF)表达的影响,为应用声动力疗法(Sonodynamic therapy,SDT)进行抗肿瘤血管新生提供实验基础。方法:种蛋随机分为4组,孵育7d后建CAM模型,随机分为空白组、单纯超声组、单纯Hp组、声动力治疗组。各组药物分别加于CAM表面的载体上,作用后制备标本,用免疫组化检测各组鸡胚CAM VEGF表达情况。结果:与空白组相比,单纯Hp组差异无统计学意义(P>0.05);单纯超声组、声动力治疗组与之差异有统计学意义(P<0.05);单纯超声组与声动力治疗组之间比较,差异有统计学意义(P<0.05)。结论:Hp介导的声动力作用显著抑制了鸡胚绒毛尿囊膜VEGF的表达,这可能是其抑制新生血管形成的重要机制。

Objective：To investigate the effect of sonodynamic therapy with hematoporphrin on the expression of VEGF in Chick Chorioallantoic Membrane and provide experimental basis for appling sonodynamic therapy（SDT） on anti-angiogenesis in tumor therapy. Methods：The hatching eggs cultured for 7 days were randomly divided into 4 groups： blank control group,ultrasound group,hematoporphrin group,sonodynamic therapy group. The express of VEGF in CAM was detected by immunity histochemistry. Result：There were significant differences between control group and ultrasound group（P〈0.05） and also between control group and SDT group（P〈0.05）,and between ultrasound group and SDT group（P〈0.05）;while no difference between control group and hematoporphrin group（P〉0.05）, conclusion The results show that sonodynamic therapy with hematoporphrin has a significant inhibitionary effect on the express of VEGF in CAM and this maybe is the most important mechanism of its anti-angiogenesis effect.