当归多糖对小鼠造血细胞表面粘附分子表达的影响

Effect of angelica polysaccharides on adhesion molecules expressions in murine hematopoietic cells

目的:探讨当归多糖(Angelica polysaccharides,APS)对小鼠造血细胞表面粘附分子表达的影响,旨在为阐述APS在造血调控和动员方面的机制提供实验依据。方法:采用外周血WBC、骨髓单个核细胞(Marraw mononuclear cell,MNC)计数,流式细胞术检测外周血和骨髓Sea-1^+细胞率以及骨髓MNC和Sca-1^+细胞表面CD49d(VLA-4的α链)、CD44表达情况。结果:1采用4mg/kg APS动员后小鼠外周血的WBC、Sca-1^+细胞数明显高于NS组(P<0.01),而骨髓MNC和Sca-1^+细胞数却明显低于NS组(P<0.01);2APS组骨髓MNC及Sca-1^+细胞表面CD49d表达明显下降(P<0.05);骨髓MNC表面CD44表达阳性率下降(P<0.05),但荧光强度无明显变化;骨髓Sca-1^+细胞表面CD44的表达与NS组无显著性差异。结论:APS可能通过降低造血细胞表面的某些粘附分子的表达而产生动员效果。

Objective ：To explore the effect of angelica polysaccharide（APS） on adhesion molecules expressions in murine hematopoietic cells,in order to provide experimental evidence of APS on hematopoietic regulation and the mechanism of mobilizing. Methods：Techniques of peripheral blood white blood cell（WBC ）and bone marrow mononuclear cells（ MNC ）count were made. Flow cytometry detected the rate of Sca-1^＋ cells in peripheral blood and bone marrow and the expression of CD49d （VLA-4 of a chain）,CD44 on bone marrow MNC and Sca-1^＋ cells. Results：l.In APS group（4mg/kg）：the number of peripheral blood WBC and Sca-1^＋ cells were markedly higher than that of NS group（P〈0.01）,and bone marrow MNC and Sca-1^＋ cells was significantly lower than that of NS group（P〈0.01）;2. In APS group：the expression of CD49d on bone marrow MNC and Sca-1^＋ cells significantly declined（P〈0.05）;the percent of CD44＇expression on bone marrow MNC also decreased（P〈0.05）, but we did not observe any change of the mean fluorescence intensity at the same time,or that of CD44＇expression on bone marrow Sca-1^＋ cells. Conclusion：APS may reduce adhesion molecules expressions on some hematopoietic cells,resulting in mobilizing hematopoietic stem and progenitor cells cells（HSPC） from the bone marrow into the peripheral blood.