右美沙芬的体外代谢方法研究

Metabolism method of dextromethorphan in vitro

目的:研究体外代谢右美沙芬的方法,并用高效液相色谱法(High performance piqid chromatography,HPLC)对右美沙芬及其产物进行测定。方法:使用正常人的肝脏匀浆在体外对右美沙芬进行代谢,用HPLC以阿霉素为内标,流动相为甲醇-10mmol/L磷酸二氢钠溶液(50:50,含0.01%庚烷磺酸钠,5%三乙胺,pH3.5),对右美沙芬及其代谢产物进行测定。结果:在适当的pH值和温度下,加入辅酶和Mg^(2+),使用人肝脏匀浆可在体外有效代谢右美沙芬,并可以用高效液相色谱法对右美沙芬及其产物进行测定。经测定,右美沙芬平均代谢率为16.09%。结论:这种体外代谢右美沙芬的方法简便灵敏,精密度高,可用于右美沙芬的体外代谢实验。

Objective：To investigate in vitro metabolism method and HPLC method for the dextromethorphan. Methods：Total protein of normal liver specimens metabolismed dextromethorphan in vitro. An HPLC method with fluorescence was developed to study the contents of dextromethorphan and metabolites by joining an internal standard（Adriamycin）. Methanol-10mmol/L sodium dihydrogen phosphate solution（50： 50,contain 0.01% sodium heptanesulfonate,5% triethylamine,pH 3.5） was used to the mobile phase.. Results：Total protein of normal liver specimens metabolismed dextromethorphan in vitro efficiently with coenzyme,Mg^2＋, suitable temperature and pH value. Dextromethorphan and metabolites were analyed by HPLC method with fluorescence. The metabolic rate of dextromethorphan was 16.09%. Conclusion：It is suggested that the metabolism method of dextromethorphan in vitro is simple,convenient and accurate. It can be used for the determination of metabolism dextromethorphan in vitro.