Visfatin转录调控的生物信息学分析

Bioinformatics analysis of the transcriptional regulation of Visfatin

目的:分析Visfatin基因可能的转录启动子区段及转录因子结合位点。方法:检索网上数据库资料,获得Visfatin的转录本信息;运用CpG/CpGReport/Isochore和FirstEF对Visfatin基因进行CpG岛分析和第一外显子预测;利用PromoterScan等程序对Visfatin的启动子进行预测。再用Matlspector、TFSEARCH和Match预测潜在的调控元件及转录因子结合位点。结果:目前已知的Visfatin转录本中,5'UTR区最长的序列为NM_005746;Visfatin基因属于典型的CpG岛相关基因,在NM_005746的5'上游229bp到798bp内很可能存在启动子,在该区域内发现了一些重要的顺式作用元件。结论:NM_005746的5'上游229bp到798bp内存在一些重要的顺式作用元件.通过实验确认这些转录因子结合位点有重要意义。

Objective：To map the approximate transcription factors binding site of Visfatin and identify its potential promoter region. Methods：All reported transcripts of Visfatin were collected through searching online database. Visfatin gene were analysed by CpG/CpGReport/lsochore and FirstEF to predict the CpG island and possible first exon,respectively.The Visfatln gDNA sequence was also analysed by softwares such as PromoterScan to predict the probable promoter region.Matlspector, TFSEARCH and Match were used to predict potential transcription factors binding sites. Results：Among all the reported transcripts of Visfatin,5＇UTR of NM_005746 Was the longest.Visfatin gene Was a typical CpG island associated gene.Promoters were predicted in she NM_005746 5＇upstream 229-798bp region.In this region,a los of cis-acting element were found. Conclusion：The transcription initiation site of Visfatin have been indentified.A lot of transcription factors binding sites were predict in the NM_005746 5＇ upstream 229 - 798bp region. It is important to definite those transcription factors binding sites through experimental methods.