蛇床子素注射液对心肺联合移植供肺保护作用的实验研究

The Experimental Research of Osthole Injection for Protective Effects of Donor Lungs in Combined Heart-Lung Transplantation

目的:研究蛇床子素注射液(Osthole injection)在心肺联合移植中对供肺的保护作用及可能机制。方法:将健康成年犬20只随机分为两组,对照组以4℃低钾右旋糖酐(Low-Potassium Dextran LPD)液灌注及保存供肺,实验组以4℃含蛇床子素注射液(40 mg/kg)的LPD液灌注及保存供肺。分别监测受体麻醉后和心肺移植后主动脉开放5 min和主动脉开放30 min的血气分析(PaO2、PaCO2),测定肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量及肺组织的湿/干重比(W/D),并观察移植肺组织的超微结构变化。结果:实验组动物PaO2高于对照组(P<0.05,P<0.01),主动脉开放5 min PaCO2明显低于对照组(P<0.05)。实验组肺组织中SOD的含量较对照组增高(P<0.01),MDA的含量较对照组降低,差异有显著性(P<0.05)。实验组较对照组的W/D低,差异有显著性(P<0.05)。电镜检查实验组的肺组织损伤明显轻于对照组。结论:蛇床子素注射液可改善移植肺的肺功能,减轻供肺损伤,增加心肺联合移植的成功率。

Objective： To study the protective effects and the possible mechanism of Osthole injection for donor lungs in combined heart-lung transplantation . Methods： Twenty adult healthy dogs were randomly divided into two groups. In the control group, the donor lungs were flushed with low-potassium dextran （LPD） solution and preserved at 4℃ while in the experimental group, the donor lungs were flushed with low-potassium dextran （LPD）solution added with Osthole injection （40 mg/kg） and preserved at 4℃, determing the analysis of blood gas PaO2 and PaCO2 of aorta declamping 5 min and 30 min of the anaesthetized receptor and the transplated heart and lung, measuring the contents of superoxide dismutase （SOD） , malon-dialdehyde （MDA） and the wet/dry ratio （W/D） in the donor lung tissues and observing the uhrastructural changes of transplanted lung tissues respectively. Results： In the experimental group , arterial blood PaO2 of animal＇s was significantly higher than that in the control group at any appointed time points （P 〈 0.05, P 〈0.01 ）. However, arterial blood PaCO2 of aorta declamping 5 min was significantly lower than that in the control group at any appointed time points （ P 〈 0.05 ）. The concentration of SOD in the lung tissues was statistically higher in the experimental group than that in the control group （P 〈 0.01 ）. The MDA concentration was lower in the experimental group than that in the control group and there were significant differences between them （P 〈 0.05 ）. The W/D in the experimental group was lower than that in the control group and there were significant differences between them （P 〈 0.05 ）. The electron microscopic examination showed the lesions in the experimental group were milder than that in the control group. Conclusion： Osthole injection can improve graft lung function , attenuate the donor lung injury significantly and increase the successful rate of combined heart-lung transplantation .