土壤细菌群体多样性的T-RFLP分析应用探讨

The application exploring of bacterial community diversity profiling in forensic comparison of soil

目的探讨土壤细菌群体多样性的末端限制性片段长度多态性(terminal restriction fragment length poly-morphism,T-RFLP)分析在法庭科学应用的相关问题。方法依据不同土壤中的细菌群体存在多样性和差异,联合利用细菌16S rDNA序列、末端限制性片段长度多态性(terminal restriction fragment length polymorphism,T-RFLP)方法对5个来源不同的土壤样品和4个同一来源土壤样品的细菌群体多样性进行比较分析,计算土壤样品间的相似系数。结果不同来源的5个土壤样品间相似系数,最大者为0.44,最小为0.3;同一来源的4个土壤样品相似系数,最大为0.87,最小为0.76。结论不同来源土壤的细菌群体多样性存在差异。

Objective To explore the application of bacterial community diversity profiling in foren- sic comparison of soil. Methods The DNA profilings of bacterial community from 5 soil samples of different source and 4 samples of same source were obtained through the specific amplification of 16S rDNA segments followed by terminal restriction fragment length polymorphism （T-RFLP） analysis, and the similarity indexes between different soil samples were calculated. Result The ranges of similarity indexes between different sources of soil samples were 0.3 - 0.44, and the ranges between same sources of soil samples were 0.76-0.87. Conclusion There is a great relativity between the bacterial community diversity profiling and the source of soil sample.