RP-HPLC法测定镇咳灵合剂中橙皮苷的含量

Detemination of the content of hesperidin in Zhenkeling mixture by RP-HPLC

目的:建立RP-HPLC法测定镇咳灵合剂中橙皮苷的含量。方法:采用Hyperail ODS2(4.6 mm×200 mm.5μm)色谱柱,流动相:乙腈-水(19:81),流速1.0 mL/min,检测波长283 nm。结果:橙皮苷在进样量为0.6328～5.0624μg/mL范围内线性关系良好,回归方程为Y=190.570X-2.789(r=0.999 9,n=6),平均加样回收率为101.10%,RSD为1.54%。结论:本方法测定简便,结果准确,重现性好,可作为制剂的质量控制。

Objective： To establish a RP-HPLC method for determination of the content of hesperidin in Zhenkeling mixture. Methods： The separation was performed on a Hypersil ODS2 （4.6 mm×200 mm, 5 μm） column. The mobile phase was acetonitrile-water （ 19：81 ）, the flow rate was 1.0 mL/min, the detecting wavelength was at 283 nm. Results： The hesperidin had a good linear relation between 0.6328 -5.0624 μg/mL （r =0.9999, n =6） , the average recovery was 101.10% , RSD was 1.54%. Conclusions： This method was simple, accurate and repeatable. It can be used for quality control in production of Zhenkeling mixture.