α-B crystallin在乳腺浸润性导管癌中的表达及意义

Expression of α-B crystallin in breast invasive ductal carcinoma

目的检测α-B crystallin在乳腺癌中的表达,并探讨其在乳腺癌中表达的意义。方法应用组织芯片及免疫组织化学法对180例乳腺浸润性导管癌、17例导管原位癌及20例正常乳腺组织中α-B crystallin以及雌激素受体(ER)、孕激素受体(PR)、HER2和Ki67的表达进行检测;根据最近提出的乳腺癌分子分型进行分组,对α-B crystallin在不同分组中的表达及与其他临床病理特征的关系行统计学分析。结果①α-Bcrystallin在乳腺导管原位癌的阳性率为41.17%(7/17),浸润性导管癌的阳性率为67.22%(121/180),两组间差异有统计学意义(P<0.05);②在浸润性导管癌中,α-B crystallin在淋巴结转移组阳性率为83.33%(81/96),无转移组阳性率为48.81%(41/84),两组间差异具有统计学意义(P<0.01,r=0.368 8);③在分子分型各组中α-B crystallin的表达差异具有统计学意义(P<0.05);其中LuminalA组与HER2+组及三阴性组两两比较差异具有统计学意义(P<0.05)。结论α-B crys-tallin在乳腺浸润性导管癌中的表达明显高于导管原位癌,与淋巴结转移呈正相关,且在生物学行为更为恶性的HER2+组和三阴性组具有更高的表达水平,提示α-B crystallin蛋白可能参与了乳腺癌的浸润与转移过程。

Objective To investigate the expression of α-B crystallin and its significance in breast carcinomas. Methods Tissue micro-array and immunohistochemical methods were used to determine the expression of α-B crystallin and other markers （ER, PR, HER2 and Ki67） in 180 cases of breast invasive ductal carcinoma （IDC）, 17 cases of ductal carcinoma in situ （DCIS） and 20 cases of normal breast tissue. These cases were grouped based on clinicopathological characteristics and molecular subtypes of breast carcinoma, and the different expressions of α-B crystallin in individual groups were evaluated. Results （1） The expressions of α-B crystallin between IDC （121/180, 67.22% ） and DCIS （7/17, 41.17 % ） were significantly different （P 〈 0.05）. （2） The expressions of α-B crystallin were strongly associated with lymph node involvement （ P 〈 0.01, r = 0.368 8）. （3） It was significantly different among those groups of the molecular subtype （ P 〈 0.05）. Among them, the P value was 0.018 2 between the luminal A and the HER2＋ groups and it was 0.008 2 between the luminal A and the triple-negative groups. Conclusion The expression of α-B crystallin in IDC is significantly higher than that in DCIS and is positively related to lymph node metastasis. There are also higher levels of expression in the HER2 ＋ and the triple-negative groups. This suggests that α-B crystallin is involved in the process of progression and metastasis of breast cancer.