母血白细胞介素6、17与胎膜早破关系的研究

A study on relationships of interleukin-6 and interleukin-17 in maternal serum with premature rupture of membranes

目的通过测定胎膜早破和非胎膜早破孕妇血中白细胞介素6、17含量,探讨白细胞介素6、17对孕足月胎膜早破合并感染的诊断价值及意义。方法酶联免疫吸附法检测胎膜早破组和对照组母血白细胞介素6、17的值。结果①胎膜早破组母血白细胞介素6、17水平与对照组比较有显著性差异(Z分别为-3.16、-3.53,P<0.01);②胎膜早破有亚临床绒毛膜羊膜炎者母血白细胞介素6、17水平与无绒毛膜羊膜炎者比较有显著性差异(t=-2.46,P<0.05;t=-3.57,P<0.01);③随着破膜时间的延长,母血和脐血白细胞介素6、17水平明显升高。破膜<12小时组与≥12小时组中母血白细胞介素6、17的比较均有显著性差异(t=-3.87,P<0.01;t=-3.38,P<0.05)。结论母血中白细胞介素6、17水平对胎膜早破合并亚临床绒毛膜羊膜炎有一定的诊断价值,且方法简单,易于临床推广。

Objective To investigate diagnostic values and significances of IL-6 and IL-17 in maternal serum for full-term premature rupture of membranes（PROM） associated with subclinical chorioamnionitis. Methods Enzyme-linked immunosorbent assay （ELISA） was used to detect IL-6 and IL-17 levels in maternal serum of women in the PROM group and the control group. Results ①The levels of IL-6 and IL-17 in maternal serum of women in the PROM group were significantly higher than those of women in the the normal control group, and the differences were significant （Z= -3.16, P 〈0.01 ; Z = -3.53, P 〈0.01 ） ; ②The levels of IL-6 and IL-17 in maternal serum in the PROM group with subclinical chorioamonitis were significantly higher than those in the PROM group without subclinical chorioamonitis （ t = - 2.46, P 〈 0.05 ; t = - 3.57, P 〈 0.01 ） ; ③The levels of IL-6 and IL-17 in maternal serum and cord blood in the PROM group were time-dependent： with prolongation of PROM time, the levels of IL-6 and IL-17 in maternal serum and cord blood were elevated significantly. In levels of IL-6 and IL-17 in maternal serum, there were significant differences between the PROM for less than 12hours group and the PROM for equal to and more than 12 hours group （ t = - 3.87, P 〈 0.01 ; t = - 3.38, P 〈 0.05）. Conclusion The levels of IL-6 and IL-17 in maternal serum have certain diagnostic values for PROM associated with subclinical chorioamnionitis, and it is a simple method and easy to be popularized in obstetric clinic.