松墨天牛成虫对幼虫虫粪挥发性物质的触角电位反应及林间驱避试验

EAG responses of Monochamus alternatus Hope (Coleoptera:Cerambycidae) to volatiles from larval frass and the repellency tests in fields

为了研制松墨天牛Monochamus alternatus Hope驱避剂,本文利用触角电位技术和田间试验的方法研究了松墨天牛雌雄成虫对幼虫虫粪挥发性物质的触角电位反应及幼虫虫粪己烷提取物、合成化合物混合物的林间驱避作用。结果表明:α-蒎烯、β-蒎烯、3-蒈烯、柠檬烯和长叶烯均能引起松墨天牛雌雄成虫一定的触角电生理反应,而4-甲基-2,6-二叔丁基苯酚不能引起松墨天牛雌雄成虫的触角电生理反应。林间驱避试验结果说明,幼虫虫粪的己烷提取物处理过的树干上的刻槽数明显少于对照树干上的刻槽数,二者具有极显著差异;有合成化合物的混合物存在的诱捕器诱捕到的雌虫数(平均值3.80±1.02)与对照诱捕到的雌虫数(平均值16.80±1.16)差异极显著。结果显示α-蒎烯、β-蒎烯、3-蒈烯、柠檬烯、长叶烯和4-甲基-2,6-二叔丁基苯酚的混合物对松墨天牛雌虫具有驱避作用,有望开发出一类新的驱避剂。

For screening a deterrent to Monochamus alternatus Hope,electroantennogram（EAG）responses of the females and males of M.alternatus to volatiles from larval frass were measured,and field tests of hexane extracts of larval frass and mixtures of each compound identified from larval frass were carried out.EAG tests showed that α-pinene,β-pinene,3-carene,limonene and longifolene all elicited EAG responses to the females and males of M.alternatus in different degrees.Butylated hydroxytoluene did not elicit EAG responses of the both sexes.Field tests showed that females gnawed significantly fewer oviposition scars on Pinus massoniana trees treated with hexane extracts of larval frass than on the control trees.Mean catch of females in traps with mixtures of synthetic compounds（3.80±1.02）was significantly fewer than those in the control traps（16.80±1.16）.These results suggested that hexane extracts of larval frass and mixtures of α-pinene,β-pinene,3-carene,limonene,longifolene and butylated hydroxytoluene can deter the females of M.alternatus and may be applied in management of this pest.