Smad通路参与ERK通路诱导血管平滑肌细胞增殖的过程

Smad pathway participates the process of proliferation of vascular smooth muscle cells induced by extracellular signal regulated kinase pathway

目的:探讨Smad通路是否参与细胞外信号调节激酶(ERK)通路诱导血管平滑肌细胞(VSMCs)增殖的过程及其可能机制。方法:将人脐动脉平滑肌细胞(hUASMCs)分为对照组、血小板源性生长因子(PDGF)组、ERK阻断剂组和PDGF+ERK阻断剂组。用MTT法测hUASMCs的增殖活性(A值),用免疫组化法测hUASMCs内细胞核增殖抗原(PCNA)、磷酸化ERK和磷酸化Smad蛋白的表达,用RT-PCR法测hUASMCs内Smad2/3mRNA的表达。结果:PDGF组hUASMCs的增殖活性(A值)及hUASMCs内的PCNA、磷酸化ERK和磷酸化Smad2/3蛋白的表达都明显高于其它各组(P<0.01);各组hUASMCs内Smad2/3mRNA的表达没有差异。结论:Smad通路可在蛋白水平参与ERK通路诱导VSMCs的增殖过程。

AIM ： To investigate whether Smad pathway participates the process of extracellular signal regulated kinase （ERK） induced the proliferation of vascular smooth muscle cells （VSMCs）. METHODS： Human umbilical artery smooth muscle cells （hUASMCs） were divided into four groups： control group, PDGF （platelet derived growth factor） group, ERK blocking agent group and PDGF ＋ ERK blocking agent group, MTT assay was used to detect the proliferation of hUASMCs （A value）, Immunohistochemical technique was used to detect the expression of PCNA, phosphorylated ERK （p- ERK） and phosphorylated Smad2/3 （p- Smad2/3） protein in hUASMCs, The expression of Smad2/3 mRNA in hUASMCs was detected by RT - PCR, RESULTS ： The proliferation of hUASMCs and the expression of PCNA, p - ERK and p - Smad2/3 proteins in hUASMCs in PDGF group were increased obviously than those in other groups （ P 〈 0. 01 ）, No difference in the expression of Smad2/3 mRNA in hUASMCs among groups was observed, CONCLUSION： Smad pathway participates the process of ERK pathway that induces the proliferation of hUASMCs at the level of protein.