益骨胶囊对去卵巢骨质疏松大鼠骨组织cbf α-1mNRA表达的影响

Effects of Yigu capsule on the level of cbf α-1 mRNA in bone of ovariectomized osteoporosis rats

目的:分析成骨细胞分化因子cbf α-1基因在去卵巢骨质疏松(OP)大鼠的表达水平,观察益骨胶囊的调节作用。方法:将36只10月龄SD雌性大鼠随机分为假手术组、模型组、药物组,相应干预后,留取股骨,采用SYBR GreenⅠ荧光定量PCR技术测定cbf α-1 mRNA表达,以GAPDH基因为内参照,根据相对定量公式(2-ΔΔCt)分别计算模型组、药物组与假手术组表达差异倍数。结果:根据相对定量公式分析显示:与假手术组相比,模型组大鼠骨组织中cbf α-1 mRNA表达下降(P<0.01)。而药物组cbf α-1 mRNA表达相当于假手术组的0.19-0.92倍(P>0.05),明显高于模型组(P<0.05)。结论:结果表明去卵巢OP模型大鼠骨组织cbf α-1基因表达降低,而益骨胶囊可上调cbf α-1的基因表达水平,这可能与其诱导骨髓间质干细胞分化为成骨细胞发挥其防治OP的作用有关。

AIM ： To analyze the regulatory effect of Yigu capsule on core binding factor alpha 1 （ cbf α - 1 ） gene expression in bone of ovariectomized osteoporosis （OP） rats. METHODS： Thirty - six 10 -month old Sprague - Dawley female rats were randomized into three groups： sham - operated group, model group and drug group. After intervention by the corresponding methods, the femurs were collected, SYBR green I fluorescence quantitative PCR technique was applied with the internal control of GAPDH according to the relative quantitative formula （2^-△△α）, the differentially expressed multiples between the model group or drug group and sham - operated group were calculated. RESULTS ： Quantitative formula analysis showed that the level of cbf α - 1 gene expression in bone of model groups was decreased than that in sham - operated rats [ compared with sham - operated group, P 〈 0.01, it was （9.9 × 105 ） - （ 1.6 × 104 ） times ]. While in drug groups the level of cbf α - 1 gene expression was 0. 19 to 0. 92 times than that in the sham - operated group, no significant difference was observed （P 〉 0. 05）. CONCLUSION. The results of this study show that cbf α - 1 gene expression in bone tissue of ovariectomized OP rat is decreased, and Yigu capsule increases the level of cbf α - 1 gene expression in bone of OP, indicating that Yigu capsule induces bone marrow mesenchymal stem cells into osteoblasts.