原卟啉二钠的抗鸭乙型肝炎病毒作用

Experimental Study on Antiviral Effect of Protoporphyrin Disodium on Duck Hepatitis B Virus

目的:研究原卟啉二钠(PPN)抗鸭乙型肝炎病毒(DHBV)的作用。方法:采用先天感染DHBV的龙岩麻鸭为动物模型,分设模型对照组、拉米呋啶组和PPN组,各组按20 mg.kg-1.d-1灌胃给药10 d,斑点杂交法观察用药前、用药5 d、10 d及停药后3 d血清中DHBV DNA表达,HE染色观察肝脏组织病理学变化。结果:PPN组于用药后5 d1、0 d,血清DHBV DNA水平分别为1.36±0.261、.05±0.28,与模型对照组2.04±0.05、1.90±0.31比较明显降低(T5P<0.05、T10P<0.01),病理检查发现PPN组肝细胞点状坏死明显减轻,与模型对照组比较,差异有显著性(P<0.05)。结论:PPN有抑制DHBV DNA的作用,并具有明显的抗炎、保肝作用。

Objective： To study the anti-duck Hepatitis B virus （DHBV） action of protoporphyrin disodium （PPN）. Methods： Longyan ducks with congenital infection of HBV were used as the animal model and the each group of ducks was given the drug with the doses 20 mg·kg^-1·d^-1 for 10 successive days. DHBV-DNA in serum was detected by dot-hybridization test before treatment, 5 days and 10 days after treatment, the pathological changes of the duck liver were also examined by HE staining. Results： After giving medication with PPN for 5 days and for 10 days, the optic density value of DHBV-DNA in ducks serum were obviously lower than that before treated （ 1.36±0. 26,1.05 ± 0. 28 VS 2.04 ± 0. 05,1.90 ± 0. 31 ）, there were remarkable difference （ P 〈 0.01, P 〈 0. 05 ）. Spotty hepatocyte necroses in treatment group was improved more obviously than that in model group. Conclusion： PPN can protect duck liver against inflammation by the inhibition of DHBV.