加味四妙汤对高尿酸血症和痛风性关节炎防治作用的实验研究

Experimental Study of the Protective Effects and Mechanisms of Additional Si-miao Decoction on Acute Gouty Arthritis and Hyperuricemia

目的:研究加味四妙汤对动物痛风性关节炎的防治作用及其作用机制。方法:采用尿酸、酵母膏分别致高尿酸血症模型,测定小鼠、大鼠血清尿酸水平、大鼠尿尿酸水平,计算尿酸排泄量;微晶型尿酸钠(MSU)诱导大鼠痛风性关节炎模型,观察大鼠步态,测定关节肿胀度、关节浸出液中TNF-α和PGE2水平。结果:加味四妙汤24,12 g.kg-1ig能明显降低高尿酸血症小鼠血清尿酸水平;12,6 g.kg-1ig能显著降低高尿酸血症大鼠血清尿酸水平,增加尿酸相对排泄量;显著抑制MSU致急性痛风性关节炎大鼠关节肿胀度,改善大鼠步态,降低关节浸出液中TNF-α、PGE2含量。结论:加味四妙汤有明显的抗高尿酸血症、防治痛风性关节炎的作用,其机制可能主要是促进尿酸排泄,抑制关节TNF-α、PGE2等因子合成与释放有关。

Objective：To explore the effects and mechanisms of Additional Si-miao Decoction on gouty arthritis and hyperuricemia in animal models. Methods：Mice were given uric acid ip or yeast extract ig to establish hyperuricemic model, serum uric acid levels were detected. Rat hyperuricemic models were made by orally administering yeast extract, uric acid in serum or urine was detected. Rat acute gouty arthritis model were established by MSU intraarticular injection, the joint swell degree,TNF-α and PGE2 in synovia were also measured. Results： Additional Si-miao Decoction 24 g·kg^-1 and 12 g·kg^-1 ig could significantly reduce the serum uric acid level in hyperuricemic mice, meanwhile, 12 g·kg^-1 and 6 g·kg^-1 ig could significantly reduce the serum uric acid level in hyperuricemic rat, together with increasing 24 hrs uric acid excretion. Additional Si-miao Decoction 12 g·kg^-1 and, 6 g·kg^-1 ig could obviously restrain the joint swelling degree in the gouty arthritis model rat and improve their gait accordingly. TNF-α and PGE2 levels in the gouty arthritis model rat were also decreased significantly after administration. Conclusions. Additional Si-miao Decoction could obviously ameliorate symptoms of hyperuricacidemia and gouty arthritis. Mechanisms of its effects may consist in accelerating the uric acid excretion and restraining the synthesis of TNF-α, PGE2 in joint.