大鼠三叉神经中脑核神经元放电滞后现象的动力学分析

Dynamic analysis of discharge hysteresis phenomenon in mesencephalic V neurons in rats

目的:利用非线性动力学理论的分析手段阐明三叉神经中脑核神经元放电滞后现象的产生机制.方法:应用脑片全细胞膜片钳技术记录大鼠三叉神经中脑核(Mes V)神经元在三角波电流刺激下的放电特征,运用动力学方法对其进行分析.结果:Ⅱ型MesV神经元放电表现出滞后现象,加入4-AP转型后滞后现象消失;非线性动力学分岔理论可以对以上现象做出解释.结论:Ⅱ型神经元的亚临界霍普夫分岔使得其存在滞后现象.

AIM： To elucidate the discharge hysteresis phenomenon in mesencephalic V （Mes V ） neurons using nonlinear dynamics theory. METHODS： Whole-cell patch clamp technique was used for firing recording and nonlinear dynamic theory was used to analyze the producing mechanism of discharge hysteresis phenomenon in Mes V neurons. RESULTS： Hysteresis of discharge was observed in type 2 Mes V neurons. After application of 4-AP, type 2 firing pattern was transferred to type 1, in which hysteresis disappeared. The nonlinear dynamic bifurcation theory could provide explanation for this phenomenon. CONCLUSION： It is the character of subcritical Andronov-Hopf bifurcation that leads to the discharge hysteresis in type 2 Mes V neurons.