葡萄糖对L02细胞LXRα与ANGPTL3基因表达的影响

Effects of glucose on LXRα and ANGPTL3 gene expressions in L02 cells

目的:研究葡萄糖对L02细胞肝X受体(LXRα)与血管生成素样蛋白3(ANGPTL3)基因表达的影响,探讨LXRα在糖尿病和脂代谢联系中的作用.方法:用5.6,7.0,11.1,28.0和33.0 mmol/L的葡萄糖培养L02细胞,5.6 mmol/L组作为对照组.用胆固醇酶联法测定各组细胞内胆固醇含量,采用RT-PCR方法检测LXRα和ANGPTL3的mRNA表达量.结果:高浓度葡萄糖可促进L02细胞内胆固醇含量上调LXRα和ANGPTL3 mRNA表达,变化差异均有统计学意义(P<0.05).结论:高浓度葡萄糖增加ANGPTL3表达,可能是通过增加细胞内总胆固醇含量而激活LXRα,而LXRα的激活则引起ANGPTL3基因的高表达.

AIM： To investigate the effects of glucose on expres- sions of LXRα and ANGPTL3 in L02 cell, and to explore the relation of LXRα with the development of diabetes mellitus accom- panied with hyperlipoproteinemia. METHODS： L02 cells were cultured separately in mediums containing 5.6, 7.0, 11.1, 28.0 and 33.0 mmol/L glucose, among which 5.6 mmol/L glucose group was taken as control. Quantitative analysis of intracellular cholesterol content was performed by cholesterol enzyme link assays. The LXRa and ANGPTL3 mRNA were determined by reverse trancriptase polymerase chain reaction （RT-PCR）. RESULTS： The expression of LXRct and ANGPTL3 mRNA increased with the increase of the glucose concentration. The levels had significant difference （ P 〈 0.05 ）. The contents of total cholesterol also increased significantly in L02 cell, and had signi- ficant difference （ P 〈 0. 05 ）. CONCLUSION： Glucose may interfere ANGPTL3 mRNA through incresasing total cholesterol, activating LXRα. The activation of LXRα upregulates the expression of ANGPTL3.