用阿托伐他汀保护急性脑缺血大鼠脑的研究

Protective effect of atorvastatin on acute cerebral ischemia injury in rats

目的:探讨阿托伐他汀对急性脑缺血大鼠的脑保护作用.方法:健康Wistar大鼠85只,雌雄不限,分为假手术组、手术组、阿托伐他汀组.给药量分别为3,8,10 mg/kg,连续灌胃14 d,于末次给药后1 h采用改良的线栓法制备大鼠大脑中动脉永久性缺血模型(pMCAO),制模成功后8 h观测各组的神经行为变化,之后处死进行红四氮唑染色观察脑梗塞体积,HE染色观察脑组织形态结构变化,免疫组织化学方法检测CD34的表达并测量微血管计数(MVC),同时检测血清总胆固醇含量.结果:阿托伐他汀给药组(3 mg/kg)大鼠脑缺血损伤程度减轻(P<0.01)、梗死灶面积减少(P<0.01),CD34阳性细胞表达增高(P<0.05),微血管计数增多(P<0.05),血清总胆固醇含量无统计学差异(P>0.05).结论:低剂量阿托伐他汀(3mg/kg)对急性脑缺血大鼠即具有脑保护作用.

AIM： To investigate the protective effects of atorvastatin On acute cerebral ischemia injury in rats. METHODS： Eightyfive healthy Wistar rats （female and male） were randomly divided into 5 groups： sham operated group, ischemic group, and atorvastatin 3, 8, 10 mg/kg groups. Rats were fed atorvastatin for 14 days successively. One hour after the last administered atorvastatin the modified way was used to prepare permanent middle cerebral artery occlusion （pMCAO） model. After 8 h of cerebral ischemia injury, the extent of neurological deficits was evaluated. The infarct area was measured by TTC staining technique. HE staining was used to observe brain tissue changes, the immunohistochemical method was used to examine CD34 expression and micro-vessel count （MVC）. The blood serum total cholesterol content was also examined. RESULTS ： In 3 mg/kg atorvastatin group the infarct size and neurological deficit scores distinctly decreased （ P 〈 0.01 ）, CD34 positive cells distinctly increased （ P 〈 0.05 ）, MCV also increased （P 〈 0.05）, but the blood serum total cholesterol content had no significant change （ P 〉 0.05）. CONCLUSION： Low dose atorvastatin has protective effects against focal cerebral ischemia in rats.