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皮肤鳞状细胞癌组织和细胞系中Ptch-1及Gli-1表达的检测

Detection of expressions of Ptch-1 and Gli-1 in squamous cell carcinoma cell lines
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摘要 目的:探讨Hedgehog信号传导通路中Ptch-1和Gli-1在鳞状细胞癌皮损和细胞系中的表达及意义.方法:采用免疫组化技术分别检测鳞状细胞癌皮损和细胞系A431,HSQ-89,HSC-2和Tca中Ptch-1和Gli-1的蛋白水平表达与分布.结果:Ptch-1及Gli-1在正常皮肤,鳞状细胞癌皮损,细胞系A431,HSQ-89以及HSC-2细胞中均有阳性表达,主要分布在鳞状细胞癌细胞胞膜及胞质中.结论:鳞状细胞癌皮损和细胞系中Ptch-1及Gli-1的阳性表达可能通过激活Hedgehog信号传导通路有助于鳞状细胞癌的形成和发展. AIM： To investigate the expressions and significance of Ptch-1 and Gli-1 in the squamous cell carcinoma lesions and the cell lines. METHODS： hnmunohistochemical techniques were employed to detect the distribution and expressions of Ptch-1 and Gli-1 in squamous cell carcinoma lesions and A431, HSQ- 89, HSC-2 and Tca squamous cell carcinoma cell lines. RESULTS： There were significant expressions of Ptch-1 and Gli-1 in the squamous cell carcinoma lesions, normal tissue and in A431, HSQ-89 and HSC-2 squamous cell carcinoma cell lines, and they were mainly distributed in the cell membrane and cytoplasma. CONCLUSION： Expressions of Ptch-1 and Gli-1 might contribute to formation and progression of squamous cell carcinoma via activating Hedgehog signaling pathway.
作者 白育萍 刘海燕 高滢 刘玉峰 李承新
机构地区 第四军医大学西京医院全军皮肤病研究所
出处 《第四军医大学学报》 北大核心 2008年第7期642-644,共3页 Journal of the Fourth Military Medical University
基金 国家自然科学基金(30572099)
关键词 Hedgehog基因 信号传导通路 免疫组化 癌 鳞状细胞 Hedgehog signal transduction pathway immuno- histochemistry carcinoma, squamous cell
分类号 R739.5 [医药卫生—肿瘤]
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参考文献9

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二级参考文献28

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第四军医大学学报
2008年 第7期

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