地高辛对模拟失重后大鼠心室功能的影响

Effects of Digoxin on Cardioventricular Function in Rats after Simulated Weightlessness

目的应用高频超声心动图观察地高辛干预尾吊模拟失重大鼠心功能变化情况,评估药物作用。方法将80只SD大鼠随机分成5组,正常对照组、悬吊组、悬吊+假饲组、悬吊+地高辛(2wk)组、悬吊+地高辛(3wk)组,每组16只。实验时间为3wk。尾吊结束后对各组其中10只大鼠行经胸超声心动图检测,6只测定血清心钠素ANP水平。结果与悬吊+假饲组相比,应用强心药物后左室收缩指标改善(P<0.01),左室收缩末期容积减小(P<0.05),血ANP水平上升(P<0.01)。给药2周组与3周组各指标差别不明显(P>0.05)。结论地高辛可增强模拟失重大鼠心肌收缩功能及射血功能,并可延缓废用性萎缩的趋势。

Objective To investigate the protective effects of Digoxin on cardioventricular function in rats after simulated weightlessness. Methods Eighty rats were divided randomly into 5 groups ： control （Con） group, tail-suspended ＋ sham feeding （TS ＋ SF） group, tail-suspended ＋ Digoxin 2 wk （TS ＋ D2） group and tail-suspended ＋ Digoxin 3 wk （TS ＋ D3） group. The TS ＋ SF group was given the distilled water. At the end of tail-suspended for 3 weeks, the heart function of 10 rats in each group was examined with echocardiography. The level of atrial natriuretic polypeptide （ANP） in serum was measured with immunoradiometric assay in other 6 rats. Results Compared with TS ＋ SF group, the contractile function of LV improved in TS ＋ D2 and TS ＋ D3 groups（P 〈0. 01 ）. The concentration of ANP increased significantly in TS ＋ D3 group.（P 〈 0.01 ）. There were no significant differences between TS ＋ D2 group and TS ＋ D3 group（P 〉0.05）. Conclusion Digoxin can improve muscle contractile and blood ejection of LV and retard the tendency of atrophy in simulated weightlessness.