加味桃核承气汤及其拆方对糖尿病血管病变的体外作用观察

In-Vitro Effect of Modified Taohe Chengqi Decoction and Its Separated Prescriptions on Diabetic Angiopathy

【目的】探讨加味桃核承气汤及其拆方对糖尿病损伤人脐静脉内皮细胞(HUVEC)培养液中组织型纤维蛋白溶酶原活化物(tPA)和纤维蛋白溶酶原活化物抑制剂-1(PAI-1)含量的影响。【方法】取纯化并鉴定后的HUVEC分10组:正常组,模型组,罗格列酮组,加味方高、中、低剂量组,活血配伍组,泻热通下配伍组,益气养阴配伍组和原方组;采用血清药理学方法,制备中药含药血清并添加至体外培养的HUVEC中,测定培养液中tPA和PAI含量,并与罗格列酮对照。【结果】与模型组比较,活血组、罗格列酮组可促进tPA含量增加(P<0.05或P<0.01),但模型组与正常组比较,tPA含量无显著性差异(P>0.05)。模型组较正常组PAI-1含量显著性升高(P<0.05);与模型组比较,加味方中、高剂量组,活血组,泻热通下组,原方组及罗格列酮组PAI-1含量均显著性降低(P<0.01)。【结论】加味桃核承气汤改善血管内皮功能的作用可能源于活血组、泻热通下组对PAI-1的调控。

Objective To investigate the effect of modified Taohe Chengqi Decoction（TCD）and its separated prescriptions on tissue plasminogen activator（tPA）and plasminogen activator inhibitor-1（PAI-1）content in human umbilical vein endothelial cells（HUVEC）with diabetic damage.Methods Purified HUVEC were divided into 10 groups： normal group,model group,rosiglitazone group,high-,middle-and low-dose modified TCD groups,blood-activating prescription group,heat-clearing and purgation prescription group,Qi-strengthening and yin-nourishing group,and TCD group.The serum containing herbal medicine was prepared through seropharmacological method,and was used for co-culture of HUVEC with high glucose and high insulin in vitro.The tPA and PAI contents in the HUVEC culture of different groups were detected.Results The content of tPA was increased in blood-activating prescription group and rosiglitazone group（P〈0.05 or P〈0.01）,and was similar in the model group with that in the normal group（P〉0.05）.The PAI-1 content in the model group was higher than that in the normal group（P〈0.01）.Compared with the model group,PAI-1 content was decreased in high-and middle-dose modified TCD groups,blood-activating prescription group,heat-clearing and purgation prescription group,rosiglitazone group,and TCD group（P〈0.01）,and the effect on PAI-1 content in the herbal medicine groups was similar to that in rosiglitazone group（P〉0.05）.Conclusion The effect of modified TCD on improving the function of endothelial cells is probably related to the regulatory effect of blood-activating,heat-clearing and purgation herbs on PAI-1.