升降散治疗急性肺损伤作用的实验研究

Experimental Study of Shengjiang Powder on Treating Acute Lung Injury

【目的】探讨升降散治疗急性肺损伤的作用机理。【方法】40只雄性SD大鼠,随机分为正常组、模型组、升降散组(剂量为2 g/kg)。给药6 d后,舌下静脉注射脂多糖(LPS)复制急性肺损伤模型,取肺组织切片,免疫组化检测;采集图像,分析微血管内皮细胞核的平均灰度。【结果】升降散组与模型组比较,能显著性抑制微血管内皮细胞核中核因子κB(NF-κB)的表达(P<0.01)。【结论】升降散可通过抑制肺微血管内皮细胞NF-κB的表达,抑制炎症反应对肺组织造成的急性损伤,对急性肺损伤起到防治作用。

Objective To investigate the therapeutic mechanism of Shengjiang Powder（SP）on acute lung injury.Methods Forty healthy SD male rats were divided into 3 groups randomly： normal group,model group,and SP group.After administration for 6 days,acute lung injury rat models were established by injecting lipopolysaccharide（LPS）into sublingual vein.Lung histological sections were prepared for the detection of NF-κB expression by immmunohistochemistry.The images of the lung histological sections were captured and the average gray values of nucleus of endothelial cells in lung micrangium were measured.Results Compared with the model group,SP could depress the expression of NF-κB in the nucleus of lung microvascular endothelial cells,and statistical difference existed between the two groups（P〈0.01）.Conclusion SP has preventive and therapeutic effect on acute lung injury by inhibiting inflammatory damage on lung tissues through depressing the expression of NF-κB in the nucleus of endothelial cells in lung micrangium.