紫外线B、臭氧、磁场协同沼泽红假单胞菌降解废水中的苯酚的效果

Degradation of phenol in wastewater by the synergetic action of UV-B, ozone, magnetic fields with Rhodopseudomonas palustris

为了提高沼泽红假单胞菌(Rhodopseudomonas palustris)在降解有机污染物过程中对开放环境的适应性,进一步提高对污染物的降解活性,分别应用紫外线B、臭氧、磁场协同沼泽红假单胞菌对苯酚溶液及炼油厂含酚废水进行降解试验,比较不同协同方式的作用效果。试验得出:在三种方式的协同降解过程中沼泽红假单胞菌的生长量、脱氢酶活性和苯酚的降解率显著高于对照。其中紫外线B协同沼泽红假单胞菌降解苯酚的处理与对照比较,菌体生长量增加了33.53%,在对数生长期的生长速率增加了10%,降解72h时其脱氢酶活性增强了31.77%。协同处理对人工废水和炼油厂含酚废水的降解效率都有显著提高。结果表明:一定强度的紫外线B、臭氧、磁场能促进沼泽红假单胞菌的生长,增强其脱氢酶活性,提高对苯酚的降解。紫外线B、臭氧、磁场协同沼泽红假单胞菌在降解苯酚过程中,沼泽红假单胞菌对开放环境的适应能力提高。

The biodegradation （as a method treating wastewater） has many advantages, such as low cost, small investment and no secondary pollution; therefore the technology of biodegradation has been focused by many researcher. The activated sludge is hard to degrade phenol completely in the wastewater. In this paper, the degradation of phenol in wastewater by the synergetic action of respective combination of UV-B, ozone, magnetic fields with Rhodopseudornonas palustris was studied. The integrated method divided into three parts. The fwst is the combination of ultraviolet-B and Rhodopseudornonas palustris（UV-BIR.p）, the second is the combination of ozone and Rhodopseudornonas palustris（O31R.p）, the third is the combination of magnetic field and Rhodopseudornonas palustris（MFIR.p）. Three methods were used to degrade phenol in wastewater. It as the technology was investigated. The factors that affect the rate of degradation were discussed. The experimental results showed that synergetic actions of three methods in the degradation were dependent on the dehydrogenase activities and the property of Rhodopseudomonas palustris. Under the experimental conditions, the growth rate and the dehydrogenase activities of microorganism and the phenol removal rate degraded by UV-B/R.p or 03/R.p or MF/R.p were higher than those of CK. Three styles of the combination could improve the degradation efficiencies obviously and similarly. For example, the growth rate and the dehydrogenase activities of Rhodopseudomonas palustris and the phenol removal rates were improved 10%, 31.77% and 33.45% respectively, degraded by UV-B/R.p. The results showed that UV-B, ozone, magnetic fields was the important factor for the enhancement of degradation efficiency of Rhodopseudomonas palustris. The growth rate and the dehydrogenase activities of Rhodopseudomonas palustris promoted by the synergetic action of respective combination of UV-B, ozone, magnetic fields with Rhodopseudomonas palustris, UV-B, O3, MF played possibly an assistant role in this process.