桃水溶性蛋白质等电聚焦电泳分析初报

ISOELECTRIC FOCUSING FOR WATER-SOLUBLE PROTEIN OF PEACH

对不同 pH 范围的两性载体电解质(Ampholine)进行等电聚焦(IEF)电泳,能获得不同的水溶性蛋白质谱型.结果表明:1.桃大多数器官酸性蛋白质含量丰富,宜选用pH3.5—7.0的 Ampholine 进行 IEF 电泳.2.桃不同器官水溶性蛋白质谱型差异显著.宜用花药、幼叶、叶柄、花瓣作为 IEF 电泳的主要分析器官;成熟叶、萼筒、种子作为辅助分析器官.3.萼筒和花瓣水溶性蛋白质谱型可鉴别黄肉桃、白肉桃两类品种,而花瓣谱型亦能区分桃大花、小花两种类型.

Isoelectric focusing(IEF)with various ampholines or various analysedorgans can obtains different patterns of water-soluble protein for peach,it is suitable to select ampholine with pH 3.5—7.0 and the organs ofanther,young leaf,petiole,petal during IEF.The patterns of water-solubleprotein of sepal tube and petal can distinguish the yellew peach and whitepeach;and the patterns of petal can differentiate non-showy flower andshowy flower of peach also.