期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

金黄色葡萄球菌胶乳凝集试验及spa基因序列改变与万古霉素耐药关系探讨 被引量：1

Study on the relationship between the changes of latex agglutination test and spa gene sequences of Staphylococcus aureus and the resistance to vancomycin
下载PDF
导出
摘要 目的研究金黄色葡萄球菌(金葡菌)胶乳凝集试验及spa基因序列改变与万古霉素耐药的关系。方法检测体外诱导对万古霉素耐药金葡菌过程中的金葡菌胶乳凝集试验结果改变情况,并利用单链构象多态性(SSCP)技术及DNA测序分析金葡菌spa基因序列有无改变。结果3株对万古霉素产生中介耐药的金葡菌在诱导过程中胶乳凝集试验发生改变,其中2株金葡菌spa基因序列有改变。结论spa基因碱基序列的改变,可能与金葡菌对万古霉素耐药有一定关联。 Objective To evaluate the changes of latex agglutination test and spa gene sequences of Staphylococcus aureus and its relationship with vancomycin resistance. Methods The latex agglutination test were detected during the inducing process of resistant strains in vitro, DNA sequences of spa gene were examined by polymerase chain reaction-sigle strand cont-ormation polymorphism（PCR-SSCP） method and were identit-ied by sequencing. Results The results of latex agglutination test in 3 strains of moderate vancomycin-resistant Staphylococcus aureus changed and the altered DNA sequences of spa gene in 2 strains were found. Conclusion The variation of the DNA sequences of spa gene is probably linked to vancomycin resistance in Staphylococcus aureus.
作者 代洪 范学工
机构地区 中南大学湘雅医院
出处 《中国感染控制杂志》 CAS 2008年第2期88-91,95,共5页 Chinese Journal of Infection Control
关键词 金黄色葡萄球菌 万古霉素 抗药性 微生物 胶乳凝集试验 spa基因 葡萄球菌蛋白A Staphylococcus aureus vancomycin drug resistance, microbial latex agglutination test spa geneStaphylococcus protein A
分类号 R378.11 [医药卫生—病原生物学]
  • 相关文献

参考文献9

  • 1代洪,范学工.金黄色葡萄球菌对万古霉素耐药情况调查[J].临床检验杂志,2005,23(4):259-262. 被引量：14
  • 2Sieradzki K, Tomasz A. Inhibition of cell wall turnover and autolysis by vancomycin in a highly vancomycin-resistant mutant of Staphylococcus aureus [J ]. J Bacteriol, 1997, 179 (8) : 2557 - 2566.
  • 3代洪,范学工.体外诱导万古霉素耐药金葡菌及耐药菌株抗生素敏感性变化分析[J].中国人兽共患病杂志,2005,21(7):598-601. 被引量：2
  • 4Tang Y W, Waddington M G, Smith D H, et al. Comparison of protein A gene sequencing with pulsed-field gel elctrophoresis and epidemiologic data for molecular typing of methicillinresi.stant Staphylococcus aureus [J]. J Clin Microbiol, 2000,38 (4):1347- 1351.
  • 5Montesinos I, Salido E, Delgado T, et al. Epidemiologic genotyping of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis at a university hospital and comparison with antibiotyplng and protein A gene and coagulase gene polymorphisms[J]. J Clin Microbiol, 2002, 40 (6):2129 - 2125.
  • 6盛小禹.DNA及RNA基本实验技术[M].北京:科学出版社,2002 216-222.
  • 7Singh V K, Schmidt J L, Jayaswal R K, et al. Impact of sigB mutation on Staphylococcus aureus oxacillin and vancomycin resistance varies with parental background and method of assessment[J]. Int J Antimicrob Agents, 2003,21 (3) : 256 - 261.
  • 8Neoh H M, Cui L Z, Yuzawa H,etal. Mutated response-regulator graR is responsible for hetero-VISA to VISA phenotypic conversion of vancomycin resistance in Staphylococcus aureus[J]. Antimicrob Agents Chemother,2008, 52(1 ):45 - 53.
  • 9McAleese F, Wu S W, Sieradzki K, et al. Over-expression of genes of the cell wall stimulon in clinical isolates of Staphylococcus aureus exhibiting cancomycin-intermediate-S, aureustype resistance to vancomycin[J]. J Bacteriol, 2006, 188(3) : 1120 - 1133.

二级参考文献22

  • 1CDC. Stapyulococcus aureus resistant to vancomycin-United States,2002[J]. MMWR,2002, 51(26):565-567.
  • 2CDC.Vancomycin-resistant Stapyulococcus aureus-Penndylvania ,2002[J].MMWR,2002,51(41):931.
  • 3CDC.Brief Report:Vancomycin resistant Staphylococcus aureus-New York,2004[J]. MMWR,2004,53(15):322-323.
  • 4Fridkin SK.Vancomycin intermediate and -resistant Staphylococcus aureus:what the infectious disease specialist needs to know[J]. Clin Infect Dis,2001,32(1):108-115.
  • 5Sieradzki K,Tomasz A.Inhibition of cell wall turnover and autolysis by vancomycin in a highly vancomycin-resistant mutant of Stapyulococcus aureus[J]. J Bacteriol,1997,179(8):2557-2566.
  • 6Cui L,Ma X,Sato K,et al.Cell wall thickening is a common feature of vancomycin resistance in Staphylococcus aureus[J]. J Clin Microbiol,2003,41(1):5-14.
  • 7van Belkum A,Bax R,Peerbooms P,et al.Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains[J].J Clin Microbiol. 1993 ,31(4):798-803.
  • 8Akineden ?,Annem?ller C,Hassan AA,et al.Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis[J].Clin Diagn Lab Immunol,2001,8(5):959-964.
  • 9Strauh JA,Hertel C,Hammes WP.A 23S RAN-targeted polymerase chain reaction-based system for detection of Staphylococcus aureus in meat starter cultures and dairy products[J].J Food Prot,1999,62(10):1150-1156.
  • 10Hiramatsu K,Aritaka N,Hanaki H,et al.Dissemination in Japanese hospitals of strains of Stapyulococcus aureus heterogeneously resistant to vancomycin[J].The Lancet,1997,350(12):1670-1673.

共引文献15

同被引文献18

引证文献1

二级引证文献5

中国感染控制杂志
2008年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部