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WFS1在大鼠胰腺不同发育阶段的表达

Expression of WFS 1 in Different Development Stages of Rat Pancreas
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摘要 目的:研究WFS1在胰腺发育不同阶段的表达和细胞定位。方法:运用Western Blot技术检测WFS1在大鼠胰腺发育不同阶段的蛋白表达水平;运用免疫荧光检测不同时期WFSl在胰腺的定位。结果:WestemBlot结果显示WFS1的蛋白表达量胚胎后期高于新生期,成年期表达量上升;免疫荧光结果显示在不同发育时期WFS1与胰岛β细胞共表达。结论:WFS1在胚胎发育中后期的高表达可能与胰岛形成及功能完善有关,并且可能参与了胰岛重塑。 To investigate the expression and cell location of WFS1 during pancreas development. Methods： The expression of in different development stages of rat pancreas was examined by Western Blot; WFS 1 location in pancreas was examined by double fluorescence immunohistochemistry. Results： The expression of WFS 1 was found much higher in the anaphase of embryo than in the newborn and was rising at the adult stage. WFS 1 and pancreatic islets cell β coexisted and were expressed in the different development stages of rat pancreas. Conclusion：The expression of WFS 1 was higher in the anaphase of embryo, which may be related to formation and func- tional improvement of pancreatic islets as well as remodeling of pancreatic islets.
作者 石慧 曹丽华 袁栎 刘莉洁 侯良芹 佟辉 王宁 德伟
机构地区 南京医科大学生物化学与分子生物学系
出处 《现代生物医学进展》 CAS 2008年第4期605-607,共3页 Progress in Modern Biomedicine
基金 国家自然科学基金(30670781) 江苏省高校自然科学基金资助(03KJR18002)
关键词 WFS1 WESTERN BLOT 免疫荧光 胰腺发育 胰岛重塑 WFS 1 Westem Blot Immunofluorescence Pancreas development Islet remodeling
分类号 Q75 [生物学—分子生物学] Q813 [生物学—生物工程]
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参考文献18

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现代生物医学进展
2008年 第4期

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