小鼠腹腔巨噬细胞的分离与培养

Separation and Cultivation of Mouse Peritoneal Macrophages

目的:建立一种小鼠腹腔巨噬细胞分离的简便方法,为低强度激光照射对巨噬细胞功能的影响研究提供实验细胞。方法:以无血清的RPMI-1640培养液灌洗小鼠腹腔,分离获取小鼠腹腔巨噬细胞,在含10%小牛血清的RPMI-1640培养液中培养。采用倒置显微镜观察细胞形态,台盼蓝染色计算存活率,瑞氏染色计算纯度。结果:获得高纯度的巨噬细胞,具备巨噬细胞的形态特征。结论:本法是一种简便实用的分离小鼠腹腔巨噬细胞的方法。

Objective： To establish a convenient method of separation and cultivation of mouse peritoneal macrophages and to provide experimental cells for investigating the effect of low-energy laser irradiation on macrophages. Methods： A mouse＇s peritoneal cavity was douched by using RPMI-1640 medium without FCS, and the mouse＇s peritoneal macrophages were separated and obtained, being cultured in RPMI-1640 medium containing 10% FCS. The morphology of the ceils was observed under inverted microscopy, the survival rate of macrophages was calculated with trypan blue stain.The purity of macrophages was analyzed by Wright＇s staining. Results： The highly purified macrophages had morphologic characteristics of the macrophage in organism. Conclusion： This method can be used for the separation of mouse peritoneal macrophages ,which is simple and easy to apply.